Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2629 | 3' | -55.9 | NC_001491.2 | + | 119347 | 0.67 | 0.85344 |
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Target: 5'- aGGAAcCG-GCUauGGCGCGCguguuGGUCAGCg -3' miRNA: 3'- -CUUUuGCaCGG--CCGCGCGg----UCAGUCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 144467 | 0.67 | 0.845473 |
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Target: 5'- -cGAGCG-GCCGGCGUuugGCCA-UgGGCg -3' miRNA: 3'- cuUUUGCaCGGCCGCG---CGGUcAgUCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 134861 | 0.67 | 0.837312 |
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Target: 5'- ---cAC-UGCaCGGCGCGCUAGUUuuauaGGCu -3' miRNA: 3'- cuuuUGcACG-GCCGCGCGGUCAG-----UCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 31844 | 0.68 | 0.828962 |
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Target: 5'- cGAAGCGgucGCCcucGGC-CGCCGG-CAGCg -3' miRNA: 3'- cUUUUGCa--CGG---CCGcGCGGUCaGUCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 90046 | 0.68 | 0.820431 |
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Target: 5'- ----cCGUGCCGGCGCucuuGCCGuuguGUCcuuGCa -3' miRNA: 3'- cuuuuGCACGGCCGCG----CGGU----CAGu--CG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 144566 | 1.12 | 0.001772 |
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Target: 5'- gGAAAACGUGCCGGCGCGCCAGUCAGCg -3' miRNA: 3'- -CUUUUGCACGGCCGCGCGGUCAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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