Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2629 | 3' | -55.9 | NC_001491.2 | + | 68539 | 0.67 | 0.868758 |
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Target: 5'- -uAGACGUGCUgggGGCGaCGCUGGcaccgacaUCGGCu -3' miRNA: 3'- cuUUUGCACGG---CCGC-GCGGUC--------AGUCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 2181 | 0.67 | 0.876099 |
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Target: 5'- cGAGACGU-CCGGgGCcCCGGUcCAGUu -3' miRNA: 3'- cUUUUGCAcGGCCgCGcGGUCA-GUCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 3685 | 0.66 | 0.896783 |
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Target: 5'- uGAAGGCGgacgGCCuGgGCGCCcuGGUCcccggAGCg -3' miRNA: 3'- -CUUUUGCa---CGGcCgCGCGG--UCAG-----UCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 95536 | 0.66 | 0.903217 |
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Target: 5'- aGAAcuCGUGCgCGcaaugguggacuGCGCGCaCAGcUCGGCu -3' miRNA: 3'- -CUUuuGCACG-GC------------CGCGCG-GUC-AGUCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 18084 | 0.66 | 0.909415 |
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Target: 5'- cAGAAUGUGCaGGgGCuGCCAGUacaAGUc -3' miRNA: 3'- cUUUUGCACGgCCgCG-CGGUCAg--UCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 2531 | 0.66 | 0.909415 |
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Target: 5'- cGAAggUGagcGCCGG-GCGCCAGgccUCGGg -3' miRNA: 3'- -CUUuuGCa--CGGCCgCGCGGUC---AGUCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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