Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2631 | 3' | -47.9 | NC_001491.2 | + | 126940 | 0.66 | 0.999225 |
Target: 5'- gGGGGCGCUa---UACCuuCGCAAGCGc -3' miRNA: 3'- -UUUCGCGGaaacAUGGuuGCGUUUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 136308 | 0.66 | 0.998823 |
Target: 5'- -uGGCgGCCUUgggcgGUGgCAGCGcCAGACGc -3' miRNA: 3'- uuUCG-CGGAAa----CAUgGUUGC-GUUUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 10281 | 0.66 | 0.998823 |
Target: 5'- -cGGCGCCggUGgagACCcACGcCGAGCu -3' miRNA: 3'- uuUCGCGGaaACa--UGGuUGC-GUUUGu -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 99821 | 0.66 | 0.998823 |
Target: 5'- ----gGCCUcUGUGCCGGCgGCAuGCAu -3' miRNA: 3'- uuucgCGGAaACAUGGUUG-CGUuUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 117040 | 0.67 | 0.997892 |
Target: 5'- uGGGGCGCgUUUGUugCAauugcucucgcGCGCcccAAGCAg -3' miRNA: 3'- -UUUCGCGgAAACAugGU-----------UGCG---UUUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 106882 | 0.67 | 0.997892 |
Target: 5'- -cGGCGCUUggGgcggagccaGCCAACGUGAACGu -3' miRNA: 3'- uuUCGCGGAaaCa--------UGGUUGCGUUUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 1575 | 0.67 | 0.996978 |
Target: 5'- -cAGCGCCgc---GCCGGcCGCAGGCGa -3' miRNA: 3'- uuUCGCGGaaacaUGGUU-GCGUUUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 147851 | 0.67 | 0.996978 |
Target: 5'- ----gGCCgcUGUACCGAUGCAuACGc -3' miRNA: 3'- uuucgCGGaaACAUGGUUGCGUuUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 83371 | 0.67 | 0.99641 |
Target: 5'- aGGAGCGgCUgcUGUACCu-CGUGAGCAa -3' miRNA: 3'- -UUUCGCgGAa-ACAUGGuuGCGUUUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 112287 | 0.68 | 0.995758 |
Target: 5'- --cGaCGCCUgUGUGguuuCCAugGCAAGCGc -3' miRNA: 3'- uuuC-GCGGAaACAU----GGUugCGUUUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 12187 | 0.68 | 0.994162 |
Target: 5'- cGAGCGCgcagcggaagacCUcgGUGCCGGCGguGGCGg -3' miRNA: 3'- uUUCGCG------------GAaaCAUGGUUGCguUUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 51195 | 0.68 | 0.9932 |
Target: 5'- aAAAGCGCCg--GcUACCAGCuguucGCAGGCc -3' miRNA: 3'- -UUUCGCGGaaaC-AUGGUUG-----CGUUUGu -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 35149 | 0.68 | 0.992115 |
Target: 5'- --uGCGCCUggGcuccGCCAgGCGCAAGCu -3' miRNA: 3'- uuuCGCGGAaaCa---UGGU-UGCGUUUGu -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 78673 | 0.69 | 0.989535 |
Target: 5'- -uAGCGcCCUUUGguauccaaaaUACCGACGCGcccAGCGg -3' miRNA: 3'- uuUCGC-GGAAAC----------AUGGUUGCGU---UUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 124704 | 0.7 | 0.977763 |
Target: 5'- --cGUGUUUUUG-GCCAACGUAGACGg -3' miRNA: 3'- uuuCGCGGAAACaUGGUUGCGUUUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 52780 | 0.7 | 0.977763 |
Target: 5'- uAAGCGCCgccgUUaUGCCAcCGUAGACGg -3' miRNA: 3'- uUUCGCGGa---AAcAUGGUuGCGUUUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 2081 | 0.7 | 0.972223 |
Target: 5'- --uGCGCCUggcgGaGCCcaGGCGCAGGCAg -3' miRNA: 3'- uuuCGCGGAaa--CaUGG--UUGCGUUUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 77361 | 0.71 | 0.96575 |
Target: 5'- cGAGGCGUCUUUGUggGCgGGCGCGccuGGCu -3' miRNA: 3'- -UUUCGCGGAAACA--UGgUUGCGU---UUGu -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 40366 | 0.71 | 0.96575 |
Target: 5'- uAAGGCGCCcccuaaUGU-CCAGCGCGuGCAu -3' miRNA: 3'- -UUUCGCGGaa----ACAuGGUUGCGUuUGU- -5' |
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2631 | 3' | -47.9 | NC_001491.2 | + | 32812 | 0.71 | 0.958284 |
Target: 5'- cGGGCGUCU----ACCAGCGCGAGCc -3' miRNA: 3'- uUUCGCGGAaacaUGGUUGCGUUUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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