Results 41 - 50 of 50 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2636 | 3' | -61.8 | NC_001491.2 | + | 2448 | 0.71 | 0.38908 |
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Target: 5'- -gGCCGCCAGCUCCccgaagcgcgcgccgUCccgGGCCgggGGGCCg -3' miRNA: 3'- agCGGUGGUCGGGG---------------AGa--CCGG---UCUGG- -5' |
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| 2636 | 3' | -61.8 | NC_001491.2 | + | 12095 | 0.71 | 0.377875 |
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Target: 5'- cUCGCCGCUggaguuggcGGCCCCcCcGGCCGcGGCUg -3' miRNA: 3'- -AGCGGUGG---------UCGGGGaGaCCGGU-CUGG- -5' |
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| 2636 | 3' | -61.8 | NC_001491.2 | + | 45572 | 0.71 | 0.354607 |
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Target: 5'- cUGCgCGCCAGCUCCUCcacaaacGGgUAGACCg -3' miRNA: 3'- aGCG-GUGGUCGGGGAGa------CCgGUCUGG- -5' |
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| 2636 | 3' | -61.8 | NC_001491.2 | + | 34519 | 0.71 | 0.347079 |
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Target: 5'- -gGCCACCAGgcuCCCCg--GGCCcugGGACCc -3' miRNA: 3'- agCGGUGGUC---GGGGagaCCGG---UCUGG- -5' |
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| 2636 | 3' | -61.8 | NC_001491.2 | + | 5305 | 0.72 | 0.31812 |
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Target: 5'- aUCGCgGCgAGCCCCg--GaGCCGGGCUg -3' miRNA: 3'- -AGCGgUGgUCGGGGagaC-CGGUCUGG- -5' |
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| 2636 | 3' | -61.8 | NC_001491.2 | + | 35798 | 0.72 | 0.31117 |
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Target: 5'- cCGCCGCCgAGCCCCgcgGGUC-GACUg -3' miRNA: 3'- aGCGGUGG-UCGGGGagaCCGGuCUGG- -5' |
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| 2636 | 3' | -61.8 | NC_001491.2 | + | 100110 | 0.72 | 0.297618 |
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Target: 5'- gUCGCUaaaACCGGCCCCg-UGGCCGuGuCCg -3' miRNA: 3'- -AGCGG---UGGUCGGGGagACCGGU-CuGG- -5' |
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| 2636 | 3' | -61.8 | NC_001491.2 | + | 2253 | 0.74 | 0.250303 |
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Target: 5'- -gGCCGCCAGCagcgccgagagucccCCCUUgaGGUCGGACCa -3' miRNA: 3'- agCGGUGGUCG---------------GGGAGa-CCGGUCUGG- -5' |
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| 2636 | 3' | -61.8 | NC_001491.2 | + | 34942 | 0.83 | 0.06143 |
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Target: 5'- cUCGgCGCgAGCCCCUCUGGUCcGACCu -3' miRNA: 3'- -AGCgGUGgUCGGGGAGACCGGuCUGG- -5' |
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| 2636 | 3' | -61.8 | NC_001491.2 | + | 137920 | 1.12 | 0.000533 |
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Target: 5'- gUCGCCACCAGCCCCUCUGGCCAGACCa -3' miRNA: 3'- -AGCGGUGGUCGGGGAGACCGGUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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