Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2639 | 3' | -52.9 | NC_001491.2 | + | 115716 | 0.66 | 0.970937 |
Target: 5'- gUCUCCCAGGUgu-UACUGGcgugcucGGCCa -3' miRNA: 3'- gAGAGGGUCCGuuuAUGGUCu------UCGGc -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 133309 | 1.1 | 0.004234 |
Target: 5'- cCUCUCCCAGGCAAAUACCAGAAGCCGg -3' miRNA: 3'- -GAGAGGGUCCGUUUAUGGUCUUCGGC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 137842 | 0.69 | 0.900066 |
Target: 5'- gUgUCCggagCAGGCugcAUGCCAGAAGUCGc -3' miRNA: 3'- gAgAGG----GUCCGuu-UAUGGUCUUCGGC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 142208 | 0.66 | 0.964613 |
Target: 5'- -cCUCCU-GGCAGG-GCCGGAAGCa- -3' miRNA: 3'- gaGAGGGuCCGUUUaUGGUCUUCGgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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