Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26432 | 5' | -63.2 | NC_005345.2 | + | 39780 | 0.67 | 0.23994 |
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Target: 5'- gCUGCCcguaGUGGUcgaGcCCGGCCg-CCCGCa -3' miRNA: 3'- aGACGGa---CACCG---C-GGUCGGagGGGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 40709 | 0.67 | 0.242355 |
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Target: 5'- -gUGCCguccGUccacaccgaccgcucGGCGuCCAGCCgcgCCCCGUc -3' miRNA: 3'- agACGGa---CA---------------CCGC-GGUCGGa--GGGGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 29316 | 0.67 | 0.246015 |
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Target: 5'- -aUGCCUGgaaGCuGUCGGCCgCCCCGg -3' miRNA: 3'- agACGGACac-CG-CGGUCGGaGGGGCg -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 20761 | 0.67 | 0.252218 |
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Target: 5'- gCUGCCgccgcgGcGGCGCguGCCcugguaUCCgCCGCc -3' miRNA: 3'- aGACGGa-----CaCCGCGguCGG------AGG-GGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 39664 | 0.67 | 0.265009 |
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Target: 5'- aCUGCCacucGGUcUCAGCCgaguagCCCCGCa -3' miRNA: 3'- aGACGGaca-CCGcGGUCGGa-----GGGGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 12301 | 0.66 | 0.277642 |
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Target: 5'- gCUGaCCUGUGcCGCCaggguccGGCCUgCaCCGCc -3' miRNA: 3'- aGAC-GGACACcGCGG-------UCGGAgG-GGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 8893 | 0.66 | 0.285173 |
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Target: 5'- -aUGCCcGccGGCGCCc-CgUCCCCGCu -3' miRNA: 3'- agACGGaCa-CCGCGGucGgAGGGGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 11208 | 0.66 | 0.292159 |
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Target: 5'- --gGCCUGUGGCaCgGGCaugggcaugaCUgCCCGCg -3' miRNA: 3'- agaCGGACACCGcGgUCG----------GAgGGGCG- -5' |
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| 26432 | 5' | -63.2 | NC_005345.2 | + | 35298 | 0.66 | 0.30653 |
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Target: 5'- -gUGgCUGUGGa--CGGCCgUCCUCGCg -3' miRNA: 3'- agACgGACACCgcgGUCGG-AGGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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