Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26441 | 5' | -56.6 | NC_005357.1 | + | 10807 | 0.66 | 0.587941 |
Target: 5'- uGCUGC-GCCUcGGcGCGgcugaauccuuCCGGCUCGGu -3' miRNA: 3'- cCGAUGuCGGAaCC-CGU-----------GGCUGAGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 32108 | 0.66 | 0.576919 |
Target: 5'- cGGCUACGGCCU--GGCgaaACUGGCgaagUGGc -3' miRNA: 3'- -CCGAUGUCGGAacCCG---UGGCUGa---GCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 6598 | 0.66 | 0.573621 |
Target: 5'- gGGCUGCGGUCUUugucgaugaccagcGGGCGCgUGGCgcugCGc -3' miRNA: 3'- -CCGAUGUCGGAA--------------CCCGUG-GCUGa---GCu -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 664 | 0.66 | 0.573621 |
Target: 5'- aGGCUGUAGCCaccaggccgccuacgGcGGCGCCGGC-CGGg -3' miRNA: 3'- -CCGAUGUCGGaa-------------C-CCGUGGCUGaGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 30844 | 0.66 | 0.555021 |
Target: 5'- cGGCcagccgGCGGCCcUGGGCGgcaUGGCUuCGAu -3' miRNA: 3'- -CCGa-----UGUCGGaACCCGUg--GCUGA-GCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 31844 | 0.66 | 0.555021 |
Target: 5'- aGGCUGCGcGCC-UGGGUACUacGCcCGAg -3' miRNA: 3'- -CCGAUGU-CGGaACCCGUGGc-UGaGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 18667 | 0.66 | 0.544163 |
Target: 5'- cGGCgcauCGGCCcgGuGGCGCCGGCaUGGc -3' miRNA: 3'- -CCGau--GUCGGaaC-CCGUGGCUGaGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 10744 | 0.66 | 0.533374 |
Target: 5'- uGCUGCucGCCaaGGGCGC--GCUCGAc -3' miRNA: 3'- cCGAUGu-CGGaaCCCGUGgcUGAGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 21960 | 0.66 | 0.5323 |
Target: 5'- uGGCUAUGGCCUUcgccgaaGGGaACCGGaaCGAg -3' miRNA: 3'- -CCGAUGUCGGAA-------CCCgUGGCUgaGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 10323 | 0.66 | 0.5323 |
Target: 5'- cGGCaGCGGCCa-GGGCguugagcaacgcgGCCGugUUGGc -3' miRNA: 3'- -CCGaUGUCGGaaCCCG-------------UGGCugAGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 36906 | 0.67 | 0.522663 |
Target: 5'- aGCUGCcGGCCUggaacuaccaGGCACCGAgUUGGc -3' miRNA: 3'- cCGAUG-UCGGAac--------CCGUGGCUgAGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 39968 | 0.67 | 0.512035 |
Target: 5'- uGCaGCAcGCCUUGugcaacaucguGGCGCCGAUcuUCGAg -3' miRNA: 3'- cCGaUGU-CGGAAC-----------CCGUGGCUG--AGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 28858 | 0.67 | 0.501498 |
Target: 5'- uGGCgagcGCGcguucaacaucGCCgUGGGCACCGAgUCa- -3' miRNA: 3'- -CCGa---UGU-----------CGGaACCCGUGGCUgAGcu -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 14416 | 0.67 | 0.491058 |
Target: 5'- gGGCgucgccagGCGcGCCUgcGGCACCGugUUGGg -3' miRNA: 3'- -CCGa-------UGU-CGGAacCCGUGGCugAGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 9911 | 0.67 | 0.491058 |
Target: 5'- cGGCaucacCAGCCUgcacGGGCGCgGAguuCUCGGc -3' miRNA: 3'- -CCGau---GUCGGAa---CCCGUGgCU---GAGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 13380 | 0.67 | 0.480719 |
Target: 5'- uGGcCUACgAGCCgccggUGGGUAUCGACcuggcCGAa -3' miRNA: 3'- -CC-GAUG-UCGGa----ACCCGUGGCUGa----GCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 1353 | 0.69 | 0.411595 |
Target: 5'- uGGUcgGCGGCCUUGcGCAuCCGGCgacCGAa -3' miRNA: 3'- -CCGa-UGUCGGAACcCGU-GGCUGa--GCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 20229 | 0.69 | 0.40223 |
Target: 5'- cGGCUguuGGCCUUGGGUGCCuuGCUCc- -3' miRNA: 3'- -CCGAug-UCGGAACCCGUGGc-UGAGcu -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 9765 | 0.69 | 0.383911 |
Target: 5'- gGGCUucacgucggGCAGCUUcGcGGCGCCGAacUUCGAc -3' miRNA: 3'- -CCGA---------UGUCGGAaC-CCGUGGCU--GAGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 14445 | 0.69 | 0.383009 |
Target: 5'- cGGCUACAGCCUgaUGGcccagccCACCGaggacguGCUCGc -3' miRNA: 3'- -CCGAUGUCGGA--ACCc------GUGGC-------UGAGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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