Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26441 | 5' | -56.6 | NC_005357.1 | + | 10744 | 0.66 | 0.533374 |
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Target: 5'- uGCUGCucGCCaaGGGCGC--GCUCGAc -3' miRNA: 3'- cCGAUGu-CGGaaCCCGUGgcUGAGCU- -5' |
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| 26441 | 5' | -56.6 | NC_005357.1 | + | 18667 | 0.66 | 0.544163 |
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Target: 5'- cGGCgcauCGGCCcgGuGGCGCCGGCaUGGc -3' miRNA: 3'- -CCGau--GUCGGaaC-CCGUGGCUGaGCU- -5' |
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| 26441 | 5' | -56.6 | NC_005357.1 | + | 31844 | 0.66 | 0.555021 |
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Target: 5'- aGGCUGCGcGCC-UGGGUACUacGCcCGAg -3' miRNA: 3'- -CCGAUGU-CGGaACCCGUGGc-UGaGCU- -5' |
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| 26441 | 5' | -56.6 | NC_005357.1 | + | 30844 | 0.66 | 0.555021 |
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Target: 5'- cGGCcagccgGCGGCCcUGGGCGgcaUGGCUuCGAu -3' miRNA: 3'- -CCGa-----UGUCGGaACCCGUg--GCUGA-GCU- -5' |
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| 26441 | 5' | -56.6 | NC_005357.1 | + | 664 | 0.66 | 0.573621 |
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Target: 5'- aGGCUGUAGCCaccaggccgccuacgGcGGCGCCGGC-CGGg -3' miRNA: 3'- -CCGAUGUCGGaa-------------C-CCGUGGCUGaGCU- -5' |
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| 26441 | 5' | -56.6 | NC_005357.1 | + | 6598 | 0.66 | 0.573621 |
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Target: 5'- gGGCUGCGGUCUUugucgaugaccagcGGGCGCgUGGCgcugCGc -3' miRNA: 3'- -CCGAUGUCGGAA--------------CCCGUG-GCUGa---GCu -5' |
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| 26441 | 5' | -56.6 | NC_005357.1 | + | 32108 | 0.66 | 0.576919 |
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Target: 5'- cGGCUACGGCCU--GGCgaaACUGGCgaagUGGc -3' miRNA: 3'- -CCGAUGUCGGAacCCG---UGGCUGa---GCU- -5' |
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| 26441 | 5' | -56.6 | NC_005357.1 | + | 10807 | 0.66 | 0.587941 |
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Target: 5'- uGCUGC-GCCUcGGcGCGgcugaauccuuCCGGCUCGGu -3' miRNA: 3'- cCGAUGuCGGAaCC-CGU-----------GGCUGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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