Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26441 | 5' | -56.6 | NC_005357.1 | + | 10323 | 0.66 | 0.5323 |
Target: 5'- cGGCaGCGGCCa-GGGCguugagcaacgcgGCCGugUUGGc -3' miRNA: 3'- -CCGaUGUCGGaaCCCG-------------UGGCugAGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 9911 | 0.67 | 0.491058 |
Target: 5'- cGGCaucacCAGCCUgcacGGGCGCgGAguuCUCGGc -3' miRNA: 3'- -CCGau---GUCGGAa---CCCGUGgCU---GAGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 9765 | 0.69 | 0.383911 |
Target: 5'- gGGCUucacgucggGCAGCUUcGcGGCGCCGAacUUCGAc -3' miRNA: 3'- -CCGA---------UGUCGGAaC-CCGUGGCU--GAGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 7448 | 0.7 | 0.340598 |
Target: 5'- cGGCUuCGGCCUucgcgcgaaUGGccuuGCACCagGACUCGAc -3' miRNA: 3'- -CCGAuGUCGGA---------ACC----CGUGG--CUGAGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 7148 | 0.75 | 0.156503 |
Target: 5'- cGGCUGCGGCCUggaugaccagggccGGGCGCUugucgGAUUCGGg -3' miRNA: 3'- -CCGAUGUCGGAa-------------CCCGUGG-----CUGAGCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 6598 | 0.66 | 0.573621 |
Target: 5'- gGGCUGCGGUCUUugucgaugaccagcGGGCGCgUGGCgcugCGc -3' miRNA: 3'- -CCGAUGUCGGAA--------------CCCGUG-GCUGa---GCu -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 1353 | 0.69 | 0.411595 |
Target: 5'- uGGUcgGCGGCCUUGcGCAuCCGGCgacCGAa -3' miRNA: 3'- -CCGa-UGUCGGAACcCGU-GGCUGa--GCU- -5' |
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26441 | 5' | -56.6 | NC_005357.1 | + | 664 | 0.66 | 0.573621 |
Target: 5'- aGGCUGUAGCCaccaggccgccuacgGcGGCGCCGGC-CGGg -3' miRNA: 3'- -CCGAUGUCGGaa-------------C-CCGUGGCUGaGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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