Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26450 | 5' | -54.9 | NC_005357.1 | + | 25031 | 0.66 | 0.645399 |
Target: 5'- -gCCGCUgaaucgaagcgGUGCGUCGGc--GCGAUGCa -3' miRNA: 3'- aaGGUGA-----------UACGCGGCCcuuCGUUGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 35689 | 0.66 | 0.645399 |
Target: 5'- uUUCCGCUAUugaGCGCgGcGGcc-CAGCGCa -3' miRNA: 3'- -AAGGUGAUA---CGCGgC-CCuucGUUGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 36251 | 0.66 | 0.640844 |
Target: 5'- --aCGCUGcggcaagaccggccUGCGCUGGGAagaugacGGCGGCGa -3' miRNA: 3'- aagGUGAU--------------ACGCGGCCCU-------UCGUUGCg -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 17700 | 0.66 | 0.634008 |
Target: 5'- -gCCGCgcagGCGCgGuGGAAGCGGCcCg -3' miRNA: 3'- aaGGUGaua-CGCGgC-CCUUCGUUGcG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 18216 | 0.66 | 0.634008 |
Target: 5'- -gCCGCU---CGCCGGacuuuGGCAGCGCc -3' miRNA: 3'- aaGGUGAuacGCGGCCcu---UCGUUGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 33051 | 0.66 | 0.634008 |
Target: 5'- -cCCGCgugcugucGUGCGCuucauCGGGucgcgcAGCGACGCg -3' miRNA: 3'- aaGGUGa-------UACGCG-----GCCCu-----UCGUUGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 15121 | 0.66 | 0.622616 |
Target: 5'- -gCCugUggGCGUCguGGGAAGCcuucgccaaGGCGCg -3' miRNA: 3'- aaGGugAuaCGCGG--CCCUUCG---------UUGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 23515 | 0.66 | 0.611233 |
Target: 5'- gUUCCGCgagcGCGCCuuccAGGCGGCGCa -3' miRNA: 3'- -AAGGUGaua-CGCGGccc-UUCGUUGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 25616 | 0.67 | 0.588534 |
Target: 5'- -aCCACg--GCGgCGcGGucgccAGCAGCGCg -3' miRNA: 3'- aaGGUGauaCGCgGC-CCu----UCGUUGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 5744 | 0.67 | 0.588534 |
Target: 5'- -aUCGCccagcGCGCCGGGcAGCGaguGCGCc -3' miRNA: 3'- aaGGUGaua--CGCGGCCCuUCGU---UGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 8991 | 0.67 | 0.577239 |
Target: 5'- uUUCCuCgccGCGCgGGGGcgcuuccagcaGGCGGCGCa -3' miRNA: 3'- -AAGGuGauaCGCGgCCCU-----------UCGUUGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 34264 | 0.67 | 0.565992 |
Target: 5'- aUCCggGCgcacGCGCaGGGAcGCAGCGCc -3' miRNA: 3'- aAGG--UGaua-CGCGgCCCUuCGUUGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 23752 | 0.67 | 0.565992 |
Target: 5'- -cCCGCgugGUGUaacagGCCGGGGgcgGGCGAcCGCc -3' miRNA: 3'- aaGGUGa--UACG-----CGGCCCU---UCGUU-GCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 19741 | 0.67 | 0.565992 |
Target: 5'- -gCUGCUuucGCGCUGGG-GGCAACuGCg -3' miRNA: 3'- aaGGUGAua-CGCGGCCCuUCGUUG-CG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 37006 | 0.67 | 0.554803 |
Target: 5'- -gCCGCcAUccGCGCCGuGGAGCGuACGCa -3' miRNA: 3'- aaGGUGaUA--CGCGGCcCUUCGU-UGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 28390 | 0.67 | 0.554803 |
Target: 5'- gUUCCAgUucGaCGCCGGcGgcGCGGCGCc -3' miRNA: 3'- -AAGGUgAuaC-GCGGCC-CuuCGUUGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 28281 | 0.67 | 0.54368 |
Target: 5'- -aCCGC---GCGCUGGGAcaCGGCGCa -3' miRNA: 3'- aaGGUGauaCGCGGCCCUucGUUGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 14488 | 0.67 | 0.53263 |
Target: 5'- aUCCcCUAcGgGUCGGGcAGCAACGg -3' miRNA: 3'- aAGGuGAUaCgCGGCCCuUCGUUGCg -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 25598 | 0.68 | 0.510783 |
Target: 5'- aUCCgaACUAUGCGCCGcGuGAAcuGCGcCGCc -3' miRNA: 3'- aAGG--UGAUACGCGGC-C-CUU--CGUuGCG- -5' |
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26450 | 5' | -54.9 | NC_005357.1 | + | 33111 | 0.68 | 0.489318 |
Target: 5'- -gCCGCgccgacGUGCGCCGccaggucgccgaGGAAGuCAAUGCg -3' miRNA: 3'- aaGGUGa-----UACGCGGC------------CCUUC-GUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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