Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26452 | 5' | -55.8 | NC_005357.1 | + | 12310 | 0.66 | 0.597476 |
Target: 5'- cGGCgUGuuCUGGcGCGGCcucAUCGCcuggGACa -3' miRNA: 3'- -CCG-ACugGACC-CGCCG---UAGCGua--UUG- -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 12267 | 0.66 | 0.575121 |
Target: 5'- cGGUagUGuCCUGGGCGG-GUUGC-UGGCc -3' miRNA: 3'- -CCG--ACuGGACCCGCCgUAGCGuAUUG- -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 32225 | 0.67 | 0.552961 |
Target: 5'- cGCcGACCaucGGGcCGGCGUgCGCuUGACg -3' miRNA: 3'- cCGaCUGGa--CCC-GCCGUA-GCGuAUUG- -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 41620 | 0.67 | 0.552961 |
Target: 5'- cGGCaaGGgCUGGGCGcggcGCGUCGCcgGAa -3' miRNA: 3'- -CCGa-CUgGACCCGC----CGUAGCGuaUUg -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 9312 | 0.67 | 0.552961 |
Target: 5'- cGGC-GACCU-GGCGGCGcacgucggCGCGgcgGGCg -3' miRNA: 3'- -CCGaCUGGAcCCGCCGUa-------GCGUa--UUG- -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 1998 | 0.67 | 0.541976 |
Target: 5'- uGGCUGAUCuUGaGGCccaGGCGcUCGC-UGGCg -3' miRNA: 3'- -CCGACUGG-AC-CCG---CCGU-AGCGuAUUG- -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 35984 | 0.67 | 0.498841 |
Target: 5'- cGGCgacGCCUucgcGGGCGGUgguGUCGCG-AGCg -3' miRNA: 3'- -CCGac-UGGA----CCCGCCG---UAGCGUaUUG- -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 10356 | 0.67 | 0.498841 |
Target: 5'- uGUUGGCg-GGGCcgaugagcauGGCGUCGCAUAGu -3' miRNA: 3'- cCGACUGgaCCCG----------CCGUAGCGUAUUg -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 27603 | 0.68 | 0.488293 |
Target: 5'- cGGCaucGACCUGGGCgaaGGCAgcgcCGCcgAGg -3' miRNA: 3'- -CCGa--CUGGACCCG---CCGUa---GCGuaUUg -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 22799 | 0.68 | 0.47785 |
Target: 5'- cGCUGGCCgaugucGCGGCc-CGCAUGACc -3' miRNA: 3'- cCGACUGGacc---CGCCGuaGCGUAUUG- -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 4531 | 0.68 | 0.447209 |
Target: 5'- uGGC-GugCUGGGCGGUcgUGCcgccGCg -3' miRNA: 3'- -CCGaCugGACCCGCCGuaGCGuau-UG- -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 37725 | 0.68 | 0.447209 |
Target: 5'- gGGCU-ACgaGGGCGGCGUCGg----- -3' miRNA: 3'- -CCGAcUGgaCCCGCCGUAGCguauug -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 29175 | 0.68 | 0.43625 |
Target: 5'- aGGCUuccucGACUUGGGCcucggugaugaccGGCAggucggacgcgCGCAUGGCg -3' miRNA: 3'- -CCGA-----CUGGACCCG-------------CCGUa----------GCGUAUUG- -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 9198 | 0.69 | 0.4274 |
Target: 5'- cGUUGGCCUGcucGGCGGCGcggCGCAc--- -3' miRNA: 3'- cCGACUGGAC---CCGCCGUa--GCGUauug -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 11850 | 0.69 | 0.417692 |
Target: 5'- cGGUUGGCCUGGGCGaaccccgcGCcaauAUCGCccAUGAg -3' miRNA: 3'- -CCGACUGGACCCGC--------CG----UAGCG--UAUUg -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 11600 | 0.69 | 0.413846 |
Target: 5'- cGGCUGGCCgccGGGCaGCAgugccagcggaaGCAUGAg -3' miRNA: 3'- -CCGACUGGa--CCCGcCGUag----------CGUAUUg -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 26269 | 0.69 | 0.408119 |
Target: 5'- cGCcGGCCUGGuCGGCucgCGCAUcGCg -3' miRNA: 3'- cCGaCUGGACCcGCCGua-GCGUAuUG- -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 13456 | 0.69 | 0.398685 |
Target: 5'- uGGC-GGCCUcgGGcGCGGUGUCggGCAUGACc -3' miRNA: 3'- -CCGaCUGGA--CC-CGCCGUAG--CGUAUUG- -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 8598 | 0.69 | 0.389392 |
Target: 5'- cGGC-GACCgugGGGaUGGCGUagGCGUGGCc -3' miRNA: 3'- -CCGaCUGGa--CCC-GCCGUAg-CGUAUUG- -5' |
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26452 | 5' | -55.8 | NC_005357.1 | + | 6703 | 0.7 | 0.380242 |
Target: 5'- aGGCacaccuUGuCCUGGGCGcGCAUCuGCuugAGCa -3' miRNA: 3'- -CCG------ACuGGACCCGC-CGUAG-CGua-UUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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