Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26461 | 5' | -59.3 | NC_005357.1 | + | 27500 | 0.74 | 0.137853 |
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Target: 5'- uGGGCGCgcaguCGGCaCuuGAGGCCGgccAGCa -3' miRNA: 3'- gCUCGUGau---GCCG-GggCUCCGGU---UCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 8422 | 0.74 | 0.120045 |
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Target: 5'- cCGuGCGCUugucuACGGCCuuGAGGCUuuGGCc -3' miRNA: 3'- -GCuCGUGA-----UGCCGGggCUCCGGu-UCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 9561 | 0.75 | 0.110411 |
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Target: 5'- uCGGGCGCgacaaucuCGGCgCCGAccgucugcGGCCAGGCg -3' miRNA: 3'- -GCUCGUGau------GCCGgGGCU--------CCGGUUCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 34734 | 0.76 | 0.098691 |
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Target: 5'- -cGGCAagcCGGCCgCCGAGGUCAAGCc -3' miRNA: 3'- gcUCGUgauGCCGG-GGCUCCGGUUCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 30091 | 0.78 | 0.070001 |
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Target: 5'- cCGGGCGCcACGGCCCUugucccaggcgauGAGGCCGcGCc -3' miRNA: 3'- -GCUCGUGaUGCCGGGG-------------CUCCGGUuCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 14995 | 0.79 | 0.055795 |
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Target: 5'- aGGGC-CUGCGGCCgCCGGGcgcggugcGCCAGGCg -3' miRNA: 3'- gCUCGuGAUGCCGG-GGCUC--------CGGUUCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 1960 | 0.8 | 0.045576 |
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Target: 5'- uCGAGCGC-GCGGCCCUGGGGgaAGGCa -3' miRNA: 3'- -GCUCGUGaUGCCGGGGCUCCggUUCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 35146 | 1.12 | 0.000182 |
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Target: 5'- gCGAGCACUACGGCCCCGAGGCCAAGCa -3' miRNA: 3'- -GCUCGUGAUGCCGGGGCUCCGGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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