Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2647 | 5' | -56.9 | NC_001491.2 | + | 27128 | 0.67 | 0.831386 |
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Target: 5'- aGCGcGUCCGC-UGCC----UCCGCCg -3' miRNA: 3'- aCGCcCAGGCGcGCGGuaaaAGGUGG- -5' |
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| 2647 | 5' | -56.9 | NC_001491.2 | + | 33481 | 0.67 | 0.839573 |
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Target: 5'- -cCGGGUCUGCGCGgCCcucgggCC-CCg -3' miRNA: 3'- acGCCCAGGCGCGC-GGuaaaa-GGuGG- -5' |
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| 2647 | 5' | -56.9 | NC_001491.2 | + | 12633 | 0.66 | 0.855384 |
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Target: 5'- gGCGGGaauuUCCcgaagGCGCGCgGggucgaCCACCg -3' miRNA: 3'- aCGCCC----AGG-----CGCGCGgUaaaa--GGUGG- -5' |
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| 2647 | 5' | -56.9 | NC_001491.2 | + | 11716 | 0.66 | 0.855384 |
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Target: 5'- gUGCugGGGUCCGCGCuGCUGgcaUCgGCg -3' miRNA: 3'- -ACG--CCCAGGCGCG-CGGUaaaAGgUGg -5' |
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| 2647 | 5' | -56.9 | NC_001491.2 | + | 147717 | 0.66 | 0.877598 |
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Target: 5'- gGCaGGGUCCGgccCGCGUCAg---CC-CCu -3' miRNA: 3'- aCG-CCCAGGC---GCGCGGUaaaaGGuGG- -5' |
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| 2647 | 5' | -56.9 | NC_001491.2 | + | 39691 | 0.66 | 0.88458 |
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Target: 5'- aGgGGGUCaGCGUGuCCGUga-CCugCa -3' miRNA: 3'- aCgCCCAGgCGCGC-GGUAaaaGGugG- -5' |
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| 2647 | 5' | -56.9 | NC_001491.2 | + | 31666 | 0.66 | 0.88458 |
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Target: 5'- gGCGaGGacccccucaUCCGCGCaGCCAgcg-CgGCCg -3' miRNA: 3'- aCGC-CC---------AGGCGCG-CGGUaaaaGgUGG- -5' |
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| 2647 | 5' | -56.9 | NC_001491.2 | + | 48381 | 0.66 | 0.889336 |
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Target: 5'- aGCGGcUCaaGCGCGCCcccggcgcgaccgUCCACCc -3' miRNA: 3'- aCGCCcAGg-CGCGCGGuaaa---------AGGUGG- -5' |
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| 2647 | 5' | -56.9 | NC_001491.2 | + | 109017 | 0.66 | 0.891341 |
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Target: 5'- aGCaGccUCGCGCGCCGUUgacaagUCCuGCCg -3' miRNA: 3'- aCGcCcaGGCGCGCGGUAAa-----AGG-UGG- -5' |
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| 2647 | 5' | -56.9 | NC_001491.2 | + | 27077 | 0.66 | 0.891341 |
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Target: 5'- aUGCGGuacaCGCGCGgCCAggccUCCGCg -3' miRNA: 3'- -ACGCCcag-GCGCGC-GGUaaa-AGGUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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