Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26498 | 3' | -57.9 | NC_005357.1 | + | 21279 | 0.7 | 0.266281 |
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Target: 5'- uUGAACCAGGgCgCGgacacGGCGGCCAaGCUGg -3' miRNA: 3'- -ACUUGGUCUgG-GU-----CCGCUGGUgCGAC- -5' |
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| 26498 | 3' | -57.9 | NC_005357.1 | + | 39574 | 0.71 | 0.245121 |
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Target: 5'- cGAACCAGGCCCcucgcuggugcuGGCGccGCUGCGCg- -3' miRNA: 3'- aCUUGGUCUGGGu-----------CCGC--UGGUGCGac -5' |
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| 26498 | 3' | -57.9 | NC_005357.1 | + | 26147 | 0.72 | 0.194073 |
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Target: 5'- -cGACCGGGuagccuUCCGGGUGGCCGCGCg- -3' miRNA: 3'- acUUGGUCU------GGGUCCGCUGGUGCGac -5' |
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| 26498 | 3' | -57.9 | NC_005357.1 | + | 10847 | 0.73 | 0.178919 |
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Target: 5'- aGAAgCAGGCCCAGGCGAgCA-GCc- -3' miRNA: 3'- aCUUgGUCUGGGUCCGCUgGUgCGac -5' |
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| 26498 | 3' | -57.9 | NC_005357.1 | + | 37069 | 0.74 | 0.160351 |
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Target: 5'- cUGGGCgCGGugC-AGGCGGCCACGCa- -3' miRNA: 3'- -ACUUG-GUCugGgUCCGCUGGUGCGac -5' |
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| 26498 | 3' | -57.9 | NC_005357.1 | + | 41438 | 0.74 | 0.147585 |
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Target: 5'- gUGAACCAcGGCaccggCCAGGCGGCCAagugGCUGc -3' miRNA: 3'- -ACUUGGU-CUG-----GGUCCGCUGGUg---CGAC- -5' |
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| 26498 | 3' | -57.9 | NC_005357.1 | + | 23133 | 1.08 | 0.000451 |
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Target: 5'- aUGAACCAGACCCAGGCGACCACGCUGu -3' miRNA: 3'- -ACUUGGUCUGGGUCCGCUGGUGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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