Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26504 | 5' | -62.4 | NC_005357.1 | + | 27609 | 0.66 | 0.28548 |
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Target: 5'- cGACCUGgGcgaaggcaGCGCCGCCgaGGUGcAGGu- -3' miRNA: 3'- -CUGGACgC--------UGCGGCGGa-CCGC-UCCuu -5' |
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| 26504 | 5' | -62.4 | NC_005357.1 | + | 33428 | 0.7 | 0.148525 |
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Target: 5'- cGCCUGCuggaaGCGCCcCCgcgcGGCGAGGAAa -3' miRNA: 3'- cUGGACGc----UGCGGcGGa---CCGCUCCUU- -5' |
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| 26504 | 5' | -62.4 | NC_005357.1 | + | 37558 | 0.72 | 0.100924 |
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Target: 5'- --aUUGCGugGCuCGCC-GGCGAGGAu -3' miRNA: 3'- cugGACGCugCG-GCGGaCCGCUCCUu -5' |
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| 26504 | 5' | -62.4 | NC_005357.1 | + | 37929 | 0.68 | 0.184195 |
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Target: 5'- cGCCUGUGGCGCCGCgCgcauccGGCGGuuucgucguucuGGAAg -3' miRNA: 3'- cUGGACGCUGCGGCG-Ga-----CCGCU------------CCUU- -5' |
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| 26504 | 5' | -62.4 | NC_005357.1 | + | 38564 | 0.69 | 0.156805 |
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Target: 5'- cGGCCUacaGCGACGgCGCCUGGUacaacuucgccGAGGu- -3' miRNA: 3'- -CUGGA---CGCUGCgGCGGACCG-----------CUCCuu -5' |
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| 26504 | 5' | -62.4 | NC_005357.1 | + | 41746 | 0.66 | 0.278464 |
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Target: 5'- cGGCCcgGcCGGCGCCGCCguaGGCGGccuGGu- -3' miRNA: 3'- -CUGGa-C-GCUGCGGCGGa--CCGCU---CCuu -5' |
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| 26504 | 5' | -62.4 | NC_005357.1 | + | 42281 | 0.7 | 0.147318 |
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Target: 5'- cGCCUGCGGgGaaGCCUGGCGccacgccguagcuuGGGAc -3' miRNA: 3'- cUGGACGCUgCggCGGACCGC--------------UCCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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