Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26504 | 5' | -62.4 | NC_005357.1 | + | 19329 | 0.66 | 0.271586 |
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Target: 5'- cGCCgUG-GGCGCCGCCguUGGCGucGGGGc -3' miRNA: 3'- cUGG-ACgCUGCGGCGG--ACCGC--UCCUu -5' |
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| 26504 | 5' | -62.4 | NC_005357.1 | + | 4785 | 0.66 | 0.278464 |
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Target: 5'- cACaCUGCGcccagcACGCCGgCUGGCGcugcGGGAu -3' miRNA: 3'- cUG-GACGC------UGCGGCgGACCGC----UCCUu -5' |
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| 26504 | 5' | -62.4 | NC_005357.1 | + | 16690 | 0.66 | 0.278464 |
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Target: 5'- cGCCcaGCGugcUGCCGCC-GGUGAGGAc -3' miRNA: 3'- cUGGa-CGCu--GCGGCGGaCCGCUCCUu -5' |
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| 26504 | 5' | -62.4 | NC_005357.1 | + | 41746 | 0.66 | 0.278464 |
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Target: 5'- cGGCCcgGcCGGCGCCGCCguaGGCGGccuGGu- -3' miRNA: 3'- -CUGGa-C-GCUGCGGCGGa--CCGCU---CCuu -5' |
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| 26504 | 5' | -62.4 | NC_005357.1 | + | 27609 | 0.66 | 0.28548 |
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Target: 5'- cGACCUGgGcgaaggcaGCGCCGCCgaGGUGcAGGu- -3' miRNA: 3'- -CUGGACgC--------UGCGGCGGa-CCGC-UCCuu -5' |
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| 26504 | 5' | -62.4 | NC_005357.1 | + | 12012 | 0.66 | 0.292634 |
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Target: 5'- cGGCCaGCaGCGCgGCCguggccGGCGAGGu- -3' miRNA: 3'- -CUGGaCGcUGCGgCGGa-----CCGCUCCuu -5' |
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| 26504 | 5' | -62.4 | NC_005357.1 | + | 5146 | 0.66 | 0.292634 |
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Target: 5'- uGCCgcgcagGCGGCcGUCGCUgcucacgcccuUGGCGAGGGu -3' miRNA: 3'- cUGGa-----CGCUG-CGGCGG-----------ACCGCUCCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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