Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26516 | 3' | -53.1 | NC_005357.1 | + | 42239 | 0.68 | 0.653452 |
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Target: 5'- cGCCCGGcagcgUGaCGGugccCAGCGAcACCGGGg -3' miRNA: 3'- -CGGGCC-----AC-GUCua--GUUGCUaUGGCUC- -5' |
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| 26516 | 3' | -53.1 | NC_005357.1 | + | 27630 | 0.68 | 0.642152 |
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Target: 5'- cGCCgaGGUGCAGGUuucCAGCGAcAUCGuGa -3' miRNA: 3'- -CGGg-CCACGUCUA---GUUGCUaUGGCuC- -5' |
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| 26516 | 3' | -53.1 | NC_005357.1 | + | 31405 | 0.71 | 0.497942 |
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Target: 5'- gGCCCGGUGCAGcagguguUCAuCGAcGCCa-- -3' miRNA: 3'- -CGGGCCACGUCu------AGUuGCUaUGGcuc -5' |
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| 26516 | 3' | -53.1 | NC_005357.1 | + | 11534 | 0.71 | 0.476879 |
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Target: 5'- aUCCGGUGCAGuGUCGGCugggauAUGCCGAc -3' miRNA: 3'- cGGGCCACGUC-UAGUUGc-----UAUGGCUc -5' |
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| 26516 | 3' | -53.1 | NC_005357.1 | + | 29795 | 0.73 | 0.379004 |
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Target: 5'- cGCCaaaGGacgGCAG--CAGCGAUGCCGAGc -3' miRNA: 3'- -CGGg--CCa--CGUCuaGUUGCUAUGGCUC- -5' |
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| 26516 | 3' | -53.1 | NC_005357.1 | + | 16619 | 1.1 | 0.001114 |
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Target: 5'- gGCCCGGUGCAGAUCAACGAUACCGAGc -3' miRNA: 3'- -CGGGCCACGUCUAGUUGCUAUGGCUC- -5' |
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| 26516 | 3' | -53.1 | NC_005357.1 | + | 37935 | 0.66 | 0.794129 |
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Target: 5'- cGCCC-GUG-AGAUUAccguCGAUACCGAc -3' miRNA: 3'- -CGGGcCACgUCUAGUu---GCUAUGGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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