Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26531 | 3' | -60.2 | NC_005357.1 | + | 41600 | 0.66 | 0.325355 |
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Target: 5'- -cUGCCCACCugGCcGA-CGUUCGGCAa -3' miRNA: 3'- uuGCGGGUGG--CGuCUaGCAGGCCGUa -5' |
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| 26531 | 3' | -60.2 | NC_005357.1 | + | 24160 | 0.67 | 0.317464 |
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Target: 5'- uGACgGCCgGCaaguucucggCGCAGGUCGUgaCCGGCAUc -3' miRNA: 3'- -UUG-CGGgUG----------GCGUCUAGCA--GGCCGUA- -5' |
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| 26531 | 3' | -60.2 | NC_005357.1 | + | 12352 | 0.67 | 0.309717 |
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Target: 5'- uGGCGCCCgggcGCCGCacaaaGGAUauuUCCGGCAc -3' miRNA: 3'- -UUGCGGG----UGGCG-----UCUAgc-AGGCCGUa -5' |
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| 26531 | 3' | -60.2 | NC_005357.1 | + | 10989 | 0.68 | 0.252917 |
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Target: 5'- cGAUGaCCaCGCCGCAGAUCGU--GGCGUc -3' miRNA: 3'- -UUGC-GG-GUGGCGUCUAGCAggCCGUA- -5' |
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| 26531 | 3' | -60.2 | NC_005357.1 | + | 30746 | 0.69 | 0.230285 |
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Target: 5'- --aGUCCGCCGCAGucugacaccuugggcAgugguguuUCGUCCGGCGUa -3' miRNA: 3'- uugCGGGUGGCGUC---------------U--------AGCAGGCCGUA- -5' |
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| 26531 | 3' | -60.2 | NC_005357.1 | + | 27831 | 0.69 | 0.216164 |
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Target: 5'- cGACGCaCUGCUgaaaaGCGGAUCGgccgCCGGCAUc -3' miRNA: 3'- -UUGCG-GGUGG-----CGUCUAGCa---GGCCGUA- -5' |
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| 26531 | 3' | -60.2 | NC_005357.1 | + | 34658 | 0.71 | 0.169672 |
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Target: 5'- gGACGCCgGC-GCAGAUCGcgCCGGUg- -3' miRNA: 3'- -UUGCGGgUGgCGUCUAGCa-GGCCGua -5' |
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| 26531 | 3' | -60.2 | NC_005357.1 | + | 5897 | 1.02 | 0.000586 |
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Target: 5'- cAACGCCCACCGCAGAUCGUCCGGCAUc -3' miRNA: 3'- -UUGCGGGUGGCGUCUAGCAGGCCGUA- -5' |
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| 26531 | 3' | -60.2 | NC_005357.1 | + | 42221 | 0.66 | 0.366978 |
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Target: 5'- uGCGCCaguuCGCCGU--GUCGcCCGGCAg -3' miRNA: 3'- uUGCGG----GUGGCGucUAGCaGGCCGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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