Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26562 | 5' | -55.6 | NC_005357.1 | + | 28241 | 0.66 | 0.600434 |
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Target: 5'- cGgGUCGGACUg--CGCCaGGCUCa- -3' miRNA: 3'- aUgCAGCUUGAacaGCGGgCCGAGcg -5' |
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| 26562 | 5' | -55.6 | NC_005357.1 | + | 28328 | 0.68 | 0.523779 |
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Target: 5'- gGCGUCGGACUcgaUGgccuuggccUCGUCgGGCagCGCg -3' miRNA: 3'- aUGCAGCUUGA---AC---------AGCGGgCCGa-GCG- -5' |
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| 26562 | 5' | -55.6 | NC_005357.1 | + | 29621 | 0.7 | 0.375418 |
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Target: 5'- aGCGcCGGgcGCUUGUgGCCgGGCUUGa -3' miRNA: 3'- aUGCaGCU--UGAACAgCGGgCCGAGCg -5' |
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| 26562 | 5' | -55.6 | NC_005357.1 | + | 32829 | 0.7 | 0.393537 |
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Target: 5'- aACGUCGAACUca-CGCCCgaGGUgCGCa -3' miRNA: 3'- aUGCAGCUUGAacaGCGGG--CCGaGCG- -5' |
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| 26562 | 5' | -55.6 | NC_005357.1 | + | 40180 | 0.71 | 0.349315 |
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Target: 5'- cGCGUCGAACUgccgGcCaagGCCCGGCguuuauacCGCg -3' miRNA: 3'- aUGCAGCUUGAa---CaG---CGGGCCGa-------GCG- -5' |
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| 26562 | 5' | -55.6 | NC_005357.1 | + | 41098 | 0.66 | 0.656176 |
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Target: 5'- cACGgcuuccUCGcGCUUGUCgGCCUGGUggccgUCGCc -3' miRNA: 3'- aUGC------AGCuUGAACAG-CGGGCCG-----AGCG- -5' |
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| 26562 | 5' | -55.6 | NC_005357.1 | + | 42211 | 0.67 | 0.544285 |
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Target: 5'- aAUGcCGAggugcgccaguucGCcgUGUCGCCCGGCagCGUg -3' miRNA: 3'- aUGCaGCU-------------UGa-ACAGCGGGCCGa-GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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