Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26562 | 5' | -55.6 | NC_005357.1 | + | 42211 | 0.67 | 0.544285 |
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Target: 5'- aAUGcCGAggugcgccaguucGCcgUGUCGCCCGGCagCGUg -3' miRNA: 3'- aUGCaGCU-------------UGa-ACAGCGGGCCGa-GCG- -5' |
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| 26562 | 5' | -55.6 | NC_005357.1 | + | 6418 | 0.68 | 0.481649 |
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Target: 5'- -uCGUCGAACUcGUC-CUCG-CUCGCg -3' miRNA: 3'- auGCAGCUUGAaCAGcGGGCcGAGCG- -5' |
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| 26562 | 5' | -55.6 | NC_005357.1 | + | 15694 | 0.69 | 0.451158 |
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Target: 5'- aGCGggCGcGCUggcgGUgGCCCGGCuUUGCa -3' miRNA: 3'- aUGCa-GCuUGAa---CAgCGGGCCG-AGCG- -5' |
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| 26562 | 5' | -55.6 | NC_005357.1 | + | 32829 | 0.7 | 0.393537 |
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Target: 5'- aACGUCGAACUca-CGCCCgaGGUgCGCa -3' miRNA: 3'- aUGCAGCUUGAacaGCGGG--CCGaGCG- -5' |
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| 26562 | 5' | -55.6 | NC_005357.1 | + | 7687 | 0.75 | 0.182333 |
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Target: 5'- -uCGUCGGGCUUGaccucggCGgCCGGCUUGCc -3' miRNA: 3'- auGCAGCUUGAACa------GCgGGCCGAGCG- -5' |
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| 26562 | 5' | -55.6 | NC_005357.1 | + | 11161 | 0.8 | 0.084921 |
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Target: 5'- cACGUCGGggucaACgaauaucacGUCGCCCGGCUCGUa -3' miRNA: 3'- aUGCAGCU-----UGaa-------CAGCGGGCCGAGCG- -5' |
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| 26562 | 5' | -55.6 | NC_005357.1 | + | 41098 | 0.66 | 0.656176 |
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Target: 5'- cACGgcuuccUCGcGCUUGUCgGCCUGGUggccgUCGCc -3' miRNA: 3'- aUGC------AGCuUGAACAG-CGGGCCG-----AGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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