Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2657 | 3' | -55.1 | NC_001491.2 | + | 133018 | 0.66 | 0.936534 |
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Target: 5'- -aCUCGCGGAgUGGGCCCgcGCCagcaaauagauUUCGGa -3' miRNA: 3'- cgGAGCGUCU-ACUUGGGa-UGG-----------GAGCC- -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 10537 | 0.66 | 0.936534 |
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Target: 5'- cGCC-CGCGGccGcaagcuCCCUcuccagcuGCCCUCGGc -3' miRNA: 3'- -CGGaGCGUCuaCuu----GGGA--------UGGGAGCC- -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 33789 | 0.66 | 0.941265 |
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Target: 5'- uCCUCGgGGAcucUG-ACCCUACCCa--- -3' miRNA: 3'- cGGAGCgUCU---ACuUGGGAUGGGagcc -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 24781 | 0.66 | 0.945762 |
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Target: 5'- cGUgaCGUGGAUgGAACgCCUACUCUgGGg -3' miRNA: 3'- -CGgaGCGUCUA-CUUG-GGAUGGGAgCC- -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 81450 | 0.66 | 0.945762 |
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Target: 5'- uGCCaaaGUAGcUGAuGCCCUggcgGCCCUCGa -3' miRNA: 3'- -CGGag-CGUCuACU-UGGGA----UGGGAGCc -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 84252 | 0.66 | 0.948772 |
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Target: 5'- aCCUagaggCGCAGAUcacagcgcuagucgGAGCCCUGgCCgCGGa -3' miRNA: 3'- cGGA-----GCGUCUA--------------CUUGGGAUgGGaGCC- -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 11947 | 0.66 | 0.950027 |
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Target: 5'- aCCUCGCuGGGUGcgacggggaaguAGCCCUGgggaCCUUGGc -3' miRNA: 3'- cGGAGCG-UCUAC------------UUGGGAUg---GGAGCC- -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 51306 | 0.66 | 0.950027 |
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Target: 5'- cGCCUuCGCGGcucUGccGGCCCcGCUCUUGGc -3' miRNA: 3'- -CGGA-GCGUCu--AC--UUGGGaUGGGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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