Results 21 - 40 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26610 | 3' | -55.5 | NC_005357.1 | + | 32811 | 0.68 | 0.503529 |
Target: 5'- cGCGACUUgaaGGCCcugaacgucgaacUCAcGCCCGAGGu -3' miRNA: 3'- cCGCUGAAg--CCGG-------------AGUuCGGGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 28897 | 0.68 | 0.494011 |
Target: 5'- aGGCGGCcgacagUUCGGCgCggacggCAAaccgcuuaccucGCCCAAGGCg -3' miRNA: 3'- -CCGCUG------AAGCCG-Ga-----GUU------------CGGGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 19223 | 0.68 | 0.494011 |
Target: 5'- cGGacaGGCUguugaagcgCGGCgUCAGGCCCAuGAa -3' miRNA: 3'- -CCg--CUGAa--------GCCGgAGUUCGGGUuCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 27427 | 0.68 | 0.494011 |
Target: 5'- uGGCGcaccGCgcccggCGGCCgCAGGCCCu-GGCg -3' miRNA: 3'- -CCGC----UGaa----GCCGGaGUUCGGGuuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 26971 | 0.68 | 0.494011 |
Target: 5'- cGGCaGCUUCGGCCaaGGGCgcgUCAAGAa -3' miRNA: 3'- -CCGcUGAAGCCGGagUUCG---GGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 14909 | 0.68 | 0.492958 |
Target: 5'- cGGCGAuCUgcugcuggcCGGCCUCAAgugccgacugcgcGCCCAAcucGGCg -3' miRNA: 3'- -CCGCU-GAa--------GCCGGAGUU-------------CGGGUU---CUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 24581 | 0.68 | 0.48249 |
Target: 5'- gGGCGACga-GGCCacgguggcgggcaUCAAcccGCCCGAGGg -3' miRNA: 3'- -CCGCUGaagCCGG-------------AGUU---CGGGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 18371 | 0.68 | 0.473161 |
Target: 5'- cGGCGGCgaggUUGGCCUUGccgauGGCgCCGGuGGCg -3' miRNA: 3'- -CCGCUGa---AGCCGGAGU-----UCG-GGUU-CUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 40414 | 0.69 | 0.446739 |
Target: 5'- cGCGACUUCgccagcgagcgccugGGCCUCAagaucAGCCacuGGCa -3' miRNA: 3'- cCGCUGAAG---------------CCGGAGU-----UCGGguuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 637 | 0.69 | 0.432856 |
Target: 5'- cGGCGuc--CGGCgUCGAgucgguGCCCAAGGCu -3' miRNA: 3'- -CCGCugaaGCCGgAGUU------CGGGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 18879 | 0.69 | 0.432856 |
Target: 5'- cGGCGAUgUCGGCCg-AGGCCaucGACc -3' miRNA: 3'- -CCGCUGaAGCCGGagUUCGGguuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 21106 | 0.69 | 0.431874 |
Target: 5'- cGGUGGC--CGGCaUCAAGCCCGAagugcucGACa -3' miRNA: 3'- -CCGCUGaaGCCGgAGUUCGGGUU-------CUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 7685 | 0.69 | 0.423095 |
Target: 5'- uGGCGGCgaucugCGGCCgguAGCCCucGAa -3' miRNA: 3'- -CCGCUGaa----GCCGGaguUCGGGuuCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 7447 | 0.69 | 0.410605 |
Target: 5'- -uCGGCUUCGGCCUUcgcgcgaauggccuuGCaCCAGGACu -3' miRNA: 3'- ccGCUGAAGCCGGAGuu-------------CG-GGUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 340 | 0.69 | 0.409654 |
Target: 5'- cGGUGGCgucgcgcagcaGGCCguccgcCAGGCCCGAGAa -3' miRNA: 3'- -CCGCUGaag--------CCGGa-----GUUCGGGUUCUg -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 35183 | 0.7 | 0.401155 |
Target: 5'- cGGCGACaUCGGCC-CGcGCCaguggccgaaguugCAGGGCu -3' miRNA: 3'- -CCGCUGaAGCCGGaGUuCGG--------------GUUCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 7247 | 0.7 | 0.394622 |
Target: 5'- cGGCGAUgUCGGCC----GCCCAGGuCu -3' miRNA: 3'- -CCGCUGaAGCCGGaguuCGGGUUCuG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 23215 | 0.7 | 0.385411 |
Target: 5'- cGGUGAaUUCGGCCUCGgccucGGCCaccGACc -3' miRNA: 3'- -CCGCUgAAGCCGGAGU-----UCGGguuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 616 | 0.7 | 0.367419 |
Target: 5'- cGGCGAUgaUGGCCUC-AGCCUc-GGCg -3' miRNA: 3'- -CCGCUGaaGCCGGAGuUCGGGuuCUG- -5' |
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26610 | 3' | -55.5 | NC_005357.1 | + | 8818 | 0.7 | 0.362135 |
Target: 5'- aGGCGguACUuggugUCGGCCUugauaccguccucgcCGacguAGCCCAGGACg -3' miRNA: 3'- -CCGC--UGA-----AGCCGGA---------------GU----UCGGGUUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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