Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26619 | 3' | -54.4 | NC_005357.1 | + | 15238 | 0.7 | 0.476928 |
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Target: 5'- gAUCAUC-AACGgcagaggGUCGGCCUGcCGCa -3' miRNA: 3'- -UAGUAGcUUGCa------UAGCCGGGCuGCGc -5' |
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| 26619 | 3' | -54.4 | NC_005357.1 | + | 23946 | 0.7 | 0.446426 |
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Target: 5'- gGUCAUCcgguGGACGaAUCGG-CCGACGCc -3' miRNA: 3'- -UAGUAG----CUUGCaUAGCCgGGCUGCGc -5' |
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| 26619 | 3' | -54.4 | NC_005357.1 | + | 39469 | 0.7 | 0.445428 |
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Target: 5'- uGUCAUCGAucACGUGcUCGacgugccGCgCGACGCGg -3' miRNA: 3'- -UAGUAGCU--UGCAU-AGC-------CGgGCUGCGC- -5' |
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| 26619 | 3' | -54.4 | NC_005357.1 | + | 9808 | 0.7 | 0.436502 |
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Target: 5'- -aCAgCGAccgcgGCGUcgCGGCCCuuGACGCGg -3' miRNA: 3'- uaGUaGCU-----UGCAuaGCCGGG--CUGCGC- -5' |
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| 26619 | 3' | -54.4 | NC_005357.1 | + | 6089 | 0.72 | 0.353301 |
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Target: 5'- aGUCGUCGAGCG---CGGCCUuGCGCa -3' miRNA: 3'- -UAGUAGCUUGCauaGCCGGGcUGCGc -5' |
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| 26619 | 3' | -54.4 | NC_005357.1 | + | 40647 | 1.06 | 0.001541 |
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Target: 5'- aAUCAUCGAACGUAUCGGCCCGACGCGc -3' miRNA: 3'- -UAGUAGCUUGCAUAGCCGGGCUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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