Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26631 | 5' | -56.6 | NC_005808.1 | + | 35216 | 0.7 | 0.34155 |
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Target: 5'- cGGCUuCGGCCUucgcgcgaaUGGccuuGCACCagGACUCGAc -3' miRNA: 3'- -CCGAuGUCGGA---------ACC----CGUGG--CUGAGCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 4320 | 0.7 | 0.34155 |
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Target: 5'- cGGCgcugACGGCC---GGCACCGACUaCGc -3' miRNA: 3'- -CCGa---UGUCGGaacCCGUGGCUGA-GCu -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 26886 | 0.71 | 0.301813 |
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Target: 5'- aGGCUgacgGCAGugugaCCUUGGGCAUCGACg--- -3' miRNA: 3'- -CCGA----UGUC-----GGAACCCGUGGCUGagcu -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 1026 | 0.74 | 0.183177 |
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Target: 5'- uGGCUGCAagcuGCCgUGGGCACCGcGCagGAc -3' miRNA: 3'- -CCGAUGU----CGGaACCCGUGGC-UGagCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 35516 | 0.75 | 0.157038 |
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Target: 5'- cGGCUGCGGCCUggaugaccagggccGGGCGCUugucgGAUUCGGg -3' miRNA: 3'- -CCGAUGUCGGAa-------------CCCGUGG-----CUGAGCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 27313 | 0.77 | 0.120666 |
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Target: 5'- cGGCcaAgAGCCUgGGGCGCCGGCUgGAc -3' miRNA: 3'- -CCGa-UgUCGGAaCCCGUGGCUGAgCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 13452 | 0.77 | 0.114009 |
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Target: 5'- aGCUugGCGGCCUcGGGCGCgGugUCGGg -3' miRNA: 3'- cCGA--UGUCGGAaCCCGUGgCugAGCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 717 | 1.1 | 0.000425 |
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Target: 5'- uGGCUACAGCCUUGGGCACCGACUCGAc -3' miRNA: 3'- -CCGAUGUCGGAACCCGUGGCUGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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