Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26631 | 5' | -56.6 | NC_005808.1 | + | 10651 | 0.66 | 0.556201 |
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Target: 5'- aGGCUGCGcGCC-UGGGUACUacGCcCGAg -3' miRNA: 3'- -CCGAUGU-CGGaACCCGUGGc-UGaGCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 10386 | 0.66 | 0.578103 |
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Target: 5'- cGGCUACGGCCU--GGCgaaACUGGCgaagUGGc -3' miRNA: 3'- -CCGAUGUCGGAacCCG---UGGCUGa---GCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 4320 | 0.7 | 0.34155 |
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Target: 5'- cGGCgcugACGGCC---GGCACCGACUaCGc -3' miRNA: 3'- -CCGa---UGUCGGaacCCGUGGCUGA-GCu -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 2526 | 0.67 | 0.513194 |
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Target: 5'- uGCaGCAcGCCUUGugcaacaucguGGCGCCGAUcuUCGAg -3' miRNA: 3'- cCGaUGU-CGGAAC-----------CCGUGGCUG--AGCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 1353 | 0.69 | 0.412654 |
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Target: 5'- uGGUcgGCGGCCUUGcGCAuCCGGCgacCGAa -3' miRNA: 3'- -CCGa-UGUCGGAACcCGU-GGCUGa--GCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 1026 | 0.74 | 0.183177 |
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Target: 5'- uGGCUGCAagcuGCCgUGGGCACCGcGCagGAc -3' miRNA: 3'- -CCGAUGU----CGGaACCCGUGGC-UGagCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 717 | 1.1 | 0.000425 |
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Target: 5'- uGGCUACAGCCUUGGGCACCGACUCGAc -3' miRNA: 3'- -CCGAUGUCGGAACCCGUGGCUGAGCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 664 | 0.66 | 0.574805 |
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Target: 5'- aGGCUGUAGCCaccaggccgccuacgGcGGCGCCGGC-CGGg -3' miRNA: 3'- -CCGAUGUCGGaa-------------C-CCGUGGCUGaGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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