Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26631 | 5' | -56.6 | NC_005808.1 | + | 10807 | 0.66 | 0.589128 |
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Target: 5'- uGCUGC-GCCUcGGcGCGgcugaauccuuCCGGCUCGGu -3' miRNA: 3'- cCGAUGuCGGAaCC-CGU-----------GGCUGAGCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 664 | 0.66 | 0.574805 |
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Target: 5'- aGGCUGUAGCCaccaggccgccuacgGcGGCGCCGGC-CGGg -3' miRNA: 3'- -CCGAUGUCGGaa-------------C-CCGUGGCUGaGCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 37075 | 0.66 | 0.564936 |
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Target: 5'- aGCUGcCGGCCUggaacuaccaaGGCACCGAgUUGGc -3' miRNA: 3'- cCGAU-GUCGGAac---------CCGUGGCUgAGCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 10744 | 0.66 | 0.534545 |
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Target: 5'- uGCUGCucGCCaaGGGCGC--GCUCGAc -3' miRNA: 3'- cCGAUGu-CGGaaCCCGUGgcUGAGCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 14416 | 0.67 | 0.492201 |
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Target: 5'- gGGCgucgccagGCGcGCCUgcGGCACCGugUUGGg -3' miRNA: 3'- -CCGa-------UGU-CGGAacCCGUGGCugAGCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 1353 | 0.69 | 0.412654 |
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Target: 5'- uGGUcgGCGGCCUUGcGCAuCCGGCgacCGAa -3' miRNA: 3'- -CCGa-UGUCGGAACcCGU-GGCUGa--GCU- -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 20229 | 0.69 | 0.403277 |
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Target: 5'- cGGCUguuGGCCUUGGGUGCCuuGCUCc- -3' miRNA: 3'- -CCGAug-UCGGAACCCGUGGc-UGAGcu -5' |
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| 26631 | 5' | -56.6 | NC_005808.1 | + | 26886 | 0.71 | 0.301813 |
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Target: 5'- aGGCUgacgGCAGugugaCCUUGGGCAUCGACg--- -3' miRNA: 3'- -CCGA----UGUC-----GGAACCCGUGGCUGagcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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