Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26639 | 5' | -65 | NC_005808.1 | + | 32787 | 0.66 | 0.20351 |
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Target: 5'- gUCCGCCCucGGCaccuCGCUgGugCUGCGc -3' miRNA: 3'- aAGGCGGG--CCGac--GCGAgCugGGCGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 35127 | 0.76 | 0.039713 |
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Target: 5'- gUgCGCUCGGCggcGUGCUCGAUCUGCGg -3' miRNA: 3'- aAgGCGGGCCGa--CGCGAGCUGGGCGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 35330 | 0.68 | 0.162794 |
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Target: 5'- -aCCGCCgagaagcugcacaaGGCcgGCGacaUCGGCCCGCGc -3' miRNA: 3'- aaGGCGGg-------------CCGa-CGCg--AGCUGGGCGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 36082 | 0.66 | 0.219673 |
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Target: 5'- aUCCGCCUcggccagggGGCUGCGgUCuuugucgauGACCaGCGg -3' miRNA: 3'- aAGGCGGG---------CCGACGCgAG---------CUGGgCGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 36329 | 0.66 | 0.214167 |
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Target: 5'- gUUCCGCCCcaGCcaaaGCGCcCGGCCCGa- -3' miRNA: 3'- -AAGGCGGGc-CGa---CGCGaGCUGGGCgc -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 36858 | 0.7 | 0.099385 |
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Target: 5'- -gCUGCCCGGC-GCGCUgggCGAUCUGUGc -3' miRNA: 3'- aaGGCGGGCCGaCGCGA---GCUGGGCGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 38226 | 0.73 | 0.06574 |
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Target: 5'- --gCGCCUGGCUGCGCaUCcGCCUGCc -3' miRNA: 3'- aagGCGGGCCGACGCG-AGcUGGGCGc -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 39859 | 0.68 | 0.148822 |
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Target: 5'- --aCGCCCGaGCaGCGC-CGGgCCGCGc -3' miRNA: 3'- aagGCGGGC-CGaCGCGaGCUgGGCGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 41872 | 0.66 | 0.2253 |
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Target: 5'- aUCCGCCag---GCGCUCGACuuggCCGUGg -3' miRNA: 3'- aAGGCGGgccgaCGCGAGCUG----GGCGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 42301 | 0.68 | 0.141122 |
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Target: 5'- -aCCaUCCGGgUGUGCUUGGCCUGCc -3' miRNA: 3'- aaGGcGGGCCgACGCGAGCUGGGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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