Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26639 | 5' | -65 | NC_005808.1 | + | 28303 | 0.66 | 0.2253 |
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Target: 5'- ---aGCCUGGC-GCaGUcCGACCCGCGc -3' miRNA: 3'- aaggCGGGCCGaCG-CGaGCUGGGCGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 11660 | 0.67 | 0.188388 |
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Target: 5'- -gCUGCCCGGCgGCcaGCcggCGGCCCuggGCGg -3' miRNA: 3'- aaGGCGGGCCGaCG--CGa--GCUGGG---CGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 24475 | 0.67 | 0.174264 |
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Target: 5'- aUCgGgCCGGCUGCGCUUcuucaACuuGCGc -3' miRNA: 3'- aAGgCgGGCCGACGCGAGc----UGggCGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 15463 | 0.68 | 0.161091 |
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Target: 5'- -gUCGUCCGGCaucuuCGC-CGGCCCGCa -3' miRNA: 3'- aaGGCGGGCCGac---GCGaGCUGGGCGc -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 10272 | 0.68 | 0.148822 |
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Target: 5'- aUCCGCCgaccaucgggcCGGCgUGCGCUUGACgUGgGa -3' miRNA: 3'- aAGGCGG-----------GCCG-ACGCGAGCUGgGCgC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 42301 | 0.68 | 0.141122 |
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Target: 5'- -aCCaUCCGGgUGUGCUUGGCCUGCc -3' miRNA: 3'- aaGGcGGGCCgACGCGAGCUGGGCGc -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 31311 | 0.71 | 0.091558 |
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Target: 5'- -gUCGCCCGGCUcGUaGCUgGGCCgCGCGc -3' miRNA: 3'- aaGGCGGGCCGA-CG-CGAgCUGG-GCGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 35127 | 0.76 | 0.039713 |
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Target: 5'- gUgCGCUCGGCggcGUGCUCGAUCUGCGg -3' miRNA: 3'- aAgGCGGGCCGa--CGCGAGCUGGGCGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 4109 | 1.05 | 0.000172 |
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Target: 5'- gUUCCGCCCGGCUGCGCUCGACCCGCGc -3' miRNA: 3'- -AAGGCGGGCCGACGCGAGCUGGGCGC- -5' |
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| 26639 | 5' | -65 | NC_005808.1 | + | 9390 | 0.66 | 0.2253 |
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Target: 5'- -gCCGCCC-GCcGCGC-CGACgUGCGc -3' miRNA: 3'- aaGGCGGGcCGaCGCGaGCUGgGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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