Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26642 | 5' | -55.8 | NC_005808.1 | + | 30186 | 0.66 | 0.598667 |
Target: 5'- cGGCgUGuuCUGGcGCGGCcucAUCGCcuggGACa -3' miRNA: 3'- -CCG-ACugGACC-CGCCG---UAGCGua--UUG- -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 11559 | 0.66 | 0.609895 |
Target: 5'- cGCUGcCCUuuGCGGUGUCGCAggcGACc -3' miRNA: 3'- cCGACuGGAccCGCCGUAGCGUa--UUG- -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 12267 | 0.66 | 0.576311 |
Target: 5'- cGGUagUGuCCUGGGCGG-GUUGC-UGGCc -3' miRNA: 3'- -CCG--ACuGGACCCGCCgUAGCGuAUUG- -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 36154 | 0.67 | 0.499996 |
Target: 5'- cGGCgacGCCUucgcGGGCGGUgguGUCGCG-AGCg -3' miRNA: 3'- -CCGac-UGGA----CCCGCCG---UAGCGUaUUG- -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 32140 | 0.67 | 0.499996 |
Target: 5'- uGUUGGCg-GGGCcgaugagcauGGCGUCGCAUAGu -3' miRNA: 3'- cCGACUGgaCCCG----------CCGUAGCGUAUUg -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 874 | 0.67 | 0.554145 |
Target: 5'- cGGCaaGGgCUGGGCGcggcGCGUCGCcgGAa -3' miRNA: 3'- -CCGa-CUgGACCCGC----CGUAGCGuaUUg -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 9312 | 0.67 | 0.554146 |
Target: 5'- cGGC-GACCU-GGCGGCGcacgucggCGCGgcgGGCg -3' miRNA: 3'- -CCGaCUGGAcCCGCCGUa-------GCGUa--UUG- -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 1998 | 0.67 | 0.543156 |
Target: 5'- uGGCUGAUCuUGaGGCccaGGCGcUCGC-UGGCg -3' miRNA: 3'- -CCGACUGG-AC-CCG---CCGU-AGCGuAUUG- -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 33916 | 0.67 | 0.543156 |
Target: 5'- cGGCgcacACCUGGGCGcGCAgcagcCGCu--GCa -3' miRNA: 3'- -CCGac--UGGACCCGC-CGUa----GCGuauUG- -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 10269 | 0.67 | 0.554145 |
Target: 5'- cGCcGACCaucGGGcCGGCGUgCGCuUGACg -3' miRNA: 3'- cCGaCUGGa--CCC-GCCGUA-GCGuAUUG- -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 37895 | 0.68 | 0.448317 |
Target: 5'- gGGCU-ACgaGGGCGGCGUCGg----- -3' miRNA: 3'- -CCGAcUGgaCCCGCCGUAGCguauug -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 19696 | 0.68 | 0.478987 |
Target: 5'- cGCUGGCCgaugucGCGGCc-CGCAUGACc -3' miRNA: 3'- cCGACUGGacc---CGCCGuaGCGUAUUG- -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 4531 | 0.68 | 0.448317 |
Target: 5'- uGGC-GugCUGGGCGGUcgUGCcgccGCg -3' miRNA: 3'- -CCGaCugGACCCGCCGuaGCGuau-UG- -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 29175 | 0.68 | 0.437345 |
Target: 5'- aGGCUuccucGACUUGGGCcucggugaugaccGGCAggucggacgcgCGCAUGGCg -3' miRNA: 3'- -CCGA-----CUGGACCCG-------------CCGUa----------GCGUAUUG- -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 14891 | 0.68 | 0.489438 |
Target: 5'- cGGCaucGACCUGGGCgaaGGCAgcgcCGCcgAGg -3' miRNA: 3'- -CCGa--CUGGACCCG---CCGUa---GCGuaUUg -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 9198 | 0.69 | 0.428484 |
Target: 5'- cGUUGGCCUGcucGGCGGCGcggCGCAc--- -3' miRNA: 3'- cCGACUGGAC---CCGCCGUa--GCGUauug -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 11850 | 0.69 | 0.418764 |
Target: 5'- cGGUUGGCCUGGGCGaaccccgcGCcaauAUCGCccAUGAg -3' miRNA: 3'- -CCGACUGGACCCGC--------CG----UAGCG--UAUUg -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 11600 | 0.69 | 0.414914 |
Target: 5'- cGGCUGGCCgccGGGCaGCAgugccagcggaaGCAUGAg -3' miRNA: 3'- -CCGACUGGa--CCCGcCGUag----------CGUAUUg -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 26269 | 0.69 | 0.409179 |
Target: 5'- cGCcGGCCUGGuCGGCucgCGCAUcGCg -3' miRNA: 3'- cCGaCUGGACCcGCCGua-GCGUAuUG- -5' |
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26642 | 5' | -55.8 | NC_005808.1 | + | 13456 | 0.69 | 0.399733 |
Target: 5'- uGGC-GGCCUcgGGcGCGGUGUCggGCAUGACc -3' miRNA: 3'- -CCGaCUGGA--CC-CGCCGUAG--CGUAUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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