Results 1 - 20 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26656 | 3' | -60.7 | NC_005808.1 | + | 402 | 0.67 | 0.309369 |
Target: 5'- gCGCCCACCagcagcACG-GCGGUgaUCGUCgGCc -3' miRNA: 3'- -GCGGGUGG------UGCuCGUCA--AGCGGgCGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 2063 | 0.7 | 0.210642 |
Target: 5'- gCGCCUgggccucaagaucaGCCACuGGCAGguggCGCCCGUa -3' miRNA: 3'- -GCGGG--------------UGGUGcUCGUCaa--GCGGGCGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 2211 | 0.66 | 0.391043 |
Target: 5'- aCGCCCucgucggguagcACCACGcgcAGCAGgcggCGCguggCCGCGc -3' miRNA: 3'- -GCGGG------------UGGUGC---UCGUCaa--GCG----GGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 2367 | 0.73 | 0.131423 |
Target: 5'- uCGgCCugCACGuGGCAGa-CGCCCGCGu -3' miRNA: 3'- -GCgGGugGUGC-UCGUCaaGCGGGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 3224 | 0.66 | 0.373625 |
Target: 5'- uGCCCGCCACGAuGaauaaUUCGaCCUGCc -3' miRNA: 3'- gCGGGUGGUGCU-Cguc--AAGC-GGGCGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 3467 | 0.71 | 0.181425 |
Target: 5'- gCGCCCcgauguUCGCGGGCgAGUUCgacgGCCCGCu -3' miRNA: 3'- -GCGGGu-----GGUGCUCG-UCAAG----CGGGCGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 3558 | 0.67 | 0.348503 |
Target: 5'- uGCgUACUgguacaGCGGGCcGUcgaacUCGCCCGCGa -3' miRNA: 3'- gCGgGUGG------UGCUCGuCA-----AGCGGGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 3795 | 0.67 | 0.332436 |
Target: 5'- uGCCCACCACcGGCGugaacggcacGggCGCCacgaGCGc -3' miRNA: 3'- gCGGGUGGUGcUCGU----------CaaGCGGg---CGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 4587 | 0.71 | 0.186278 |
Target: 5'- cCGCCCAgcacgccacguUCACGGGCGc--CGCCCGUGa -3' miRNA: 3'- -GCGGGU-----------GGUGCUCGUcaaGCGGGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 4898 | 0.67 | 0.332436 |
Target: 5'- gGCCUcguuuucgGCCACGguggaaacgucGGCAGcgaUGCCCGCGa -3' miRNA: 3'- gCGGG--------UGGUGC-----------UCGUCaa-GCGGGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 5535 | 0.67 | 0.332436 |
Target: 5'- cCGCCCAgguuCCGCc--CAGgUCGCCCGCc -3' miRNA: 3'- -GCGGGU----GGUGcucGUCaAGCGGGCGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 6740 | 0.67 | 0.324609 |
Target: 5'- gGgCCGCCGCGcucaauAGCggaaaggucuuuAGUUUGCCCGCc -3' miRNA: 3'- gCgGGUGGUGC------UCG------------UCAAGCGGGCGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 8599 | 0.7 | 0.201527 |
Target: 5'- gGCCUgcgauACCugG-GCaAGUUCGCCgGCGa -3' miRNA: 3'- gCGGG-----UGGugCuCG-UCAAGCGGgCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 9265 | 0.67 | 0.340401 |
Target: 5'- gCGCCgCGCCGcCGAGCAGgccaacgUCGaCgGCGc -3' miRNA: 3'- -GCGG-GUGGU-GCUCGUCa------AGCgGgCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 9623 | 0.69 | 0.243823 |
Target: 5'- uGCUCGCCaccgGCGAGCAGaUCGCacuggccgaacagggCCGCa -3' miRNA: 3'- gCGGGUGG----UGCUCGUCaAGCG---------------GGCGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 9723 | 0.67 | 0.309369 |
Target: 5'- aCGCCUcgaaGCCGCGGGCGaacUGCUCGUGg -3' miRNA: 3'- -GCGGG----UGGUGCUCGUcaaGCGGGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 9802 | 1.09 | 0.000232 |
Target: 5'- gCGCCCACCACGAGCAGUUCGCCCGCGg -3' miRNA: 3'- -GCGGGUGGUGCUCGUCAAGCGGGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 10924 | 0.68 | 0.279848 |
Target: 5'- uGCCCGCCGguuugcagggccuCGGGCAGUU-GCgCgGCGa -3' miRNA: 3'- gCGGGUGGU-------------GCUCGUCAAgCG-GgCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 10985 | 0.66 | 0.356741 |
Target: 5'- gGCCCugCaaaccgGCGGGCAGgUCGCCauuccgGUGg -3' miRNA: 3'- gCGGGugG------UGCUCGUCaAGCGGg-----CGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 11125 | 0.67 | 0.309369 |
Target: 5'- gGUUCAaacgUGCGAGCAGUUCGgCCUGCu -3' miRNA: 3'- gCGGGUg---GUGCUCGUCAAGC-GGGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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