Results 1 - 20 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26656 | 3' | -60.7 | NC_005808.1 | + | 6740 | 0.67 | 0.324609 |
Target: 5'- gGgCCGCCGCGcucaauAGCggaaaggucuuuAGUUUGCCCGCc -3' miRNA: 3'- gCgGGUGGUGC------UCG------------UCAAGCGGGCGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 24774 | 0.68 | 0.301956 |
Target: 5'- uGaCCUGCCACGGGUcGUccUCgauGCCCGCGu -3' miRNA: 3'- gC-GGGUGGUGCUCGuCA--AG---CGGGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 26430 | 0.68 | 0.301956 |
Target: 5'- uGCCCucgACCuCGuGCAGUUCGCggaUGCGc -3' miRNA: 3'- gCGGG---UGGuGCuCGUCAAGCGg--GCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 11241 | 0.68 | 0.301956 |
Target: 5'- aCGCCCACUAgCGuGGCgaugagGGUUUGCgCUGCGg -3' miRNA: 3'- -GCGGGUGGU-GC-UCG------UCAAGCG-GGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 36159 | 0.68 | 0.301956 |
Target: 5'- aCGCCU-UCGCGGGCGGUggugUCGCgaGCGa -3' miRNA: 3'- -GCGGGuGGUGCUCGUCA----AGCGggCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 11125 | 0.67 | 0.309369 |
Target: 5'- gGUUCAaacgUGCGAGCAGUUCGgCCUGCu -3' miRNA: 3'- gCGGGUg---GUGCUCGUCAAGC-GGGCGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 402 | 0.67 | 0.309369 |
Target: 5'- gCGCCCACCagcagcACG-GCGGUgaUCGUCgGCc -3' miRNA: 3'- -GCGGGUGG------UGCuCGUCA--AGCGGgCGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 9723 | 0.67 | 0.309369 |
Target: 5'- aCGCCUcgaaGCCGCGGGCGaacUGCUCGUGg -3' miRNA: 3'- -GCGGG----UGGUGCUCGUcaaGCGGGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 31719 | 0.67 | 0.324609 |
Target: 5'- aGCCCGCCGCGcGCGGgcuuuuucaCGCCUaaaaauauGCGc -3' miRNA: 3'- gCGGGUGGUGCuCGUCaa-------GCGGG--------CGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 27252 | 0.68 | 0.29468 |
Target: 5'- gCGCCCcaggcucuugGCCgaggcaAUGAaccGCAGUUCGCCgCGCGc -3' miRNA: 3'- -GCGGG----------UGG------UGCU---CGUCAAGCGG-GCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 22510 | 0.68 | 0.29468 |
Target: 5'- uGCCCGCCaucauccccgcaGCGAGCAcgcgcagCGCgCGCGu -3' miRNA: 3'- gCGGGUGG------------UGCUCGUcaa----GCGgGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 15995 | 0.68 | 0.287542 |
Target: 5'- gGUgCGCCGCGcGCAGggacaggUCGCCgGUGa -3' miRNA: 3'- gCGgGUGGUGCuCGUCa------AGCGGgCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 2367 | 0.73 | 0.131423 |
Target: 5'- uCGgCCugCACGuGGCAGa-CGCCCGCGu -3' miRNA: 3'- -GCgGGugGUGC-UCGUCaaGCGGGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 24673 | 0.73 | 0.131423 |
Target: 5'- gCGCCCGCCGCGGGCcacGUccaCGCCCagGCu -3' miRNA: 3'- -GCGGGUGGUGCUCGu--CAa--GCGGG--CGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 3467 | 0.71 | 0.181425 |
Target: 5'- gCGCCCcgauguUCGCGGGCgAGUUCgacgGCCCGCu -3' miRNA: 3'- -GCGGGu-----GGUGCUCG-UCAAG----CGGGCGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 35590 | 0.71 | 0.181425 |
Target: 5'- aCGCCCAUCacGCGAGCcGaccCGCUCGCGc -3' miRNA: 3'- -GCGGGUGG--UGCUCGuCaa-GCGGGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 12667 | 0.71 | 0.191244 |
Target: 5'- uCGCCCAUCAUGcGCAGgcgcgugUCgagGCCCGCc -3' miRNA: 3'- -GCGGGUGGUGCuCGUCa------AG---CGGGCGc -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 13495 | 0.71 | 0.193264 |
Target: 5'- uGCCgACCGCGccuuugggcgagguaAGCGGUUugcCGUCCGCGc -3' miRNA: 3'- gCGGgUGGUGC---------------UCGUCAA---GCGGGCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 25642 | 0.68 | 0.273675 |
Target: 5'- gCGUCCACCuCG-GCgAGUUCuGCCgGCGu -3' miRNA: 3'- -GCGGGUGGuGCuCG-UCAAG-CGGgCGC- -5' |
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26656 | 3' | -60.7 | NC_005808.1 | + | 10924 | 0.68 | 0.279848 |
Target: 5'- uGCCCGCCGguuugcagggccuCGGGCAGUU-GCgCgGCGa -3' miRNA: 3'- gCGGGUGGU-------------GCUCGUCAAgCG-GgCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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