Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26684 | 5' | -55.3 | NC_005808.1 | + | 1656 | 0.67 | 0.61082 |
Target: 5'- gUCUgcgcGUCCuGUUUGCGCGUGA--UGCUGc -3' miRNA: 3'- -AGA----UAGG-CAAGCGCGCGCUgaGCGAC- -5' |
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26684 | 5' | -55.3 | NC_005808.1 | + | 5617 | 0.66 | 0.644298 |
Target: 5'- --gGUgCGuUUCGCGCGUGgcgcggcgcaGCUUGCUGg -3' miRNA: 3'- agaUAgGC-AAGCGCGCGC----------UGAGCGAC- -5' |
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26684 | 5' | -55.3 | NC_005808.1 | + | 7606 | 0.67 | 0.581933 |
Target: 5'- gUCUGUCCGUcugcCGUggugauggucaggggGCGCGGCUCGa-- -3' miRNA: 3'- -AGAUAGGCAa---GCG---------------CGCGCUGAGCgac -5' |
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26684 | 5' | -55.3 | NC_005808.1 | + | 11082 | 0.7 | 0.402103 |
Target: 5'- -gUGUCCGggUCGCGCGCGAgcacCUUgucgGCUGc -3' miRNA: 3'- agAUAGGCa-AGCGCGCGCU----GAG----CGAC- -5' |
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26684 | 5' | -55.3 | NC_005808.1 | + | 15125 | 0.7 | 0.430707 |
Target: 5'- ---cUCgCGUggugCGUGCGCGGCgCGCUGg -3' miRNA: 3'- agauAG-GCAa---GCGCGCGCUGaGCGAC- -5' |
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26684 | 5' | -55.3 | NC_005808.1 | + | 16300 | 0.79 | 0.119571 |
Target: 5'- cCUGUCCGUugauggcauccaUCGCGCGCGAUgCGCg- -3' miRNA: 3'- aGAUAGGCA------------AGCGCGCGCUGaGCGac -5' |
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26684 | 5' | -55.3 | NC_005808.1 | + | 20064 | 0.7 | 0.430707 |
Target: 5'- ----aCCGgcaCGCGCGCGcugcgcguGCUCGCUGc -3' miRNA: 3'- agauaGGCaa-GCGCGCGC--------UGAGCGAC- -5' |
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26684 | 5' | -55.3 | NC_005808.1 | + | 22068 | 0.67 | 0.566489 |
Target: 5'- cUCggGUCgCGgUCgGCGCGCGGCUCGaUGg -3' miRNA: 3'- -AGa-UAG-GCaAG-CGCGCGCUGAGCgAC- -5' |
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26684 | 5' | -55.3 | NC_005808.1 | + | 24432 | 0.66 | 0.633137 |
Target: 5'- aUUUGUCgGUgcCGCGCGCGGcCUCGg-- -3' miRNA: 3'- -AGAUAGgCAa-GCGCGCGCU-GAGCgac -5' |
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26684 | 5' | -55.3 | NC_005808.1 | + | 24821 | 1.08 | 0.000905 |
Target: 5'- uUCUAUCCGUUCGCGCGCGACUCGCUGc -3' miRNA: 3'- -AGAUAGGCAAGCGCGCGCUGAGCGAC- -5' |
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26684 | 5' | -55.3 | NC_005808.1 | + | 34984 | 0.68 | 0.544617 |
Target: 5'- -gUGUCCGg-CGCuGCGCGACcacgUGCUGu -3' miRNA: 3'- agAUAGGCaaGCG-CGCGCUGa---GCGAC- -5' |
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26684 | 5' | -55.3 | NC_005808.1 | + | 41543 | 0.67 | 0.588579 |
Target: 5'- aCUGgacgCCgGUUCGCG-GCGACaUGCUGc -3' miRNA: 3'- aGAUa---GG-CAAGCGCgCGCUGaGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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