Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26692 | 3' | -50.6 | NC_005808.1 | + | 34815 | 0.66 | 0.88872 |
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Target: 5'- gGCGGCgaagGGUGgaCGcCGGcgcagaucgcgccGGUGCUGCa -3' miRNA: 3'- -CGUUGa---CCACgaGCuGUU-------------CUACGACG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 35359 | 0.69 | 0.730261 |
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Target: 5'- gGCGGCcgacaucgccgagGGUGagcgCGGCGcAGAUGCUGCc -3' miRNA: 3'- -CGUUGa------------CCACga--GCUGU-UCUACGACG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 38745 | 0.67 | 0.799056 |
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Target: 5'- -aAGCUGGg---CGGCGAGGUGC-GCa -3' miRNA: 3'- cgUUGACCacgaGCUGUUCUACGaCG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 39535 | 0.69 | 0.700825 |
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Target: 5'- uGCGGCUcgGGcGCUUGAC---GUGCUGCu -3' miRNA: 3'- -CGUUGA--CCaCGAGCUGuucUACGACG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 40384 | 0.67 | 0.799056 |
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Target: 5'- uGCGcgUGGUGCUaccCGACGAGG-GCgucgGCa -3' miRNA: 3'- -CGUugACCACGA---GCUGUUCUaCGa---CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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