Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26692 | 3' | -50.6 | NC_005808.1 | + | 5191 | 0.66 | 0.847422 |
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Target: 5'- uGUAuuuGCUGGUGCUgGu---GGUGCUGg -3' miRNA: 3'- -CGU---UGACCACGAgCuguuCUACGACg -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 13748 | 0.66 | 0.865073 |
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Target: 5'- cGCGgcGCUGGgacaGCuUCGGCAAGuccgGCcGCa -3' miRNA: 3'- -CGU--UGACCa---CG-AGCUGUUCua--CGaCG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 19481 | 0.66 | 0.873495 |
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Target: 5'- uGCGcCaGGUGCcggUgGGCAGGccGCUGCg -3' miRNA: 3'- -CGUuGaCCACG---AgCUGUUCuaCGACG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 14436 | 0.66 | 0.873495 |
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Target: 5'- gGCAACgGcGUGgUCGaucugaccaccgGCAAGcUGCUGCc -3' miRNA: 3'- -CGUUGaC-CACgAGC------------UGUUCuACGACG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 34815 | 0.66 | 0.88872 |
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Target: 5'- gGCGGCgaagGGUGgaCGcCGGcgcagaucgcgccGGUGCUGCa -3' miRNA: 3'- -CGUUGa---CCACgaGCuGUU-------------CUACGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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