miRNA: 3'- cGUUGACCa----CGaGCUGUUCUACGA-CG- -5'
miRNA: 3'- -CGUUGacCACGAGCUGuUCUACGA-CG- -5'
miRNA: 3'- -CGUU-GACCACGAgCUGUUCua-CGACG- -5'
miRNA: 3'- -CGU-UGACCACga--GCU-GUUCUACGACG- -5'
miRNA: 3'- -CGUUGACCACGAGCUGUUCUACGACG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26692 | 3' | -50.6 | NC_005808.1 | + | 31496 | 0.72 | 0.527817 |
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Target: 5'- cCAAUUGGccgcaGC-CGACAAGGUGCUcGCg -3' miRNA: 3'- cGUUGACCa----CGaGCUGUUCUACGA-CG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 33022 | 0.74 | 0.451834 |
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Target: 5'- cGCAAC--GUGUUCGACcGGAUGCUcGCc -3' miRNA: 3'- -CGUUGacCACGAGCUGuUCUACGA-CG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 8594 | 0.77 | 0.280611 |
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Target: 5'- uGCGGcCUGGUGCUgGAUAAGcugGUUGCg -3' miRNA: 3'- -CGUU-GACCACGAgCUGUUCua-CGACG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 32527 | 0.78 | 0.24506 |
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Target: 5'- uGCAgGCUGGUGaugcCGGcCAGGAUGCUGCc -3' miRNA: 3'- -CGU-UGACCACga--GCU-GUUCUACGACG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 22554 | 1.14 | 0.000882 |
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Target: 5'- cGCAACUGGUGCUCGACAAGAUGCUGCc -3' miRNA: 3'- -CGUUGACCACGAGCUGUUCUACGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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