Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26693 | 3' | -58.3 | NC_005808.1 | + | 32116 | 0.67 | 0.393653 |
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Target: 5'- cGGCCCGUUcggGCcGGUGCugcgcgacuuccaGGGCGACGcCa -3' miRNA: 3'- -UUGGGCGA---UGaCCACG-------------CCCGUUGCuG- -5' |
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| 26693 | 3' | -58.3 | NC_005808.1 | + | 39660 | 0.67 | 0.403856 |
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Target: 5'- cGugCCGCgcgACgcGGUGUGGGC--CGGCa -3' miRNA: 3'- -UugGGCGa--UGa-CCACGCCCGuuGCUG- -5' |
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| 26693 | 3' | -58.3 | NC_005808.1 | + | 34198 | 0.66 | 0.422829 |
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Target: 5'- uGCUCGCUGCccgacacGUGGGCGGCGAUu -3' miRNA: 3'- uUGGGCGAUGacca---CGCCCGUUGCUG- -5' |
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| 26693 | 3' | -58.3 | NC_005808.1 | + | 2353 | 0.66 | 0.422829 |
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Target: 5'- cGAUCUGUUGCUGG-GCaaacGGCAACGGu -3' miRNA: 3'- -UUGGGCGAUGACCaCGc---CCGUUGCUg -5' |
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| 26693 | 3' | -58.3 | NC_005808.1 | + | 13539 | 0.66 | 0.436425 |
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Target: 5'- uGACCCGCUGaUGGccuacgagccgccgGUGGGUAuCGACc -3' miRNA: 3'- -UUGGGCGAUgACCa-------------CGCCCGUuGCUG- -5' |
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| 26693 | 3' | -58.3 | NC_005808.1 | + | 9315 | 0.66 | 0.452266 |
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Target: 5'- cGACCUgGCgGCgcacgucGGcgcgGCGGGCGGCGGCa -3' miRNA: 3'- -UUGGG-CGaUGa------CCa---CGCCCGUUGCUG- -5' |
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| 26693 | 3' | -58.3 | NC_005808.1 | + | 30369 | 0.66 | 0.461313 |
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Target: 5'- gAACCCGCcgcUGCUGuaggGUGGGUcggugauAACGGCg -3' miRNA: 3'- -UUGGGCG---AUGACca--CGCCCG-------UUGCUG- -5' |
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| 26693 | 3' | -58.3 | NC_005808.1 | + | 17521 | 0.66 | 0.462324 |
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Target: 5'- gGGCgCGCUgcGCUGGgugGCGaugcugcccgauGGUAACGGCg -3' miRNA: 3'- -UUGgGCGA--UGACCa--CGC------------CCGUUGCUG- -5' |
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| 26693 | 3' | -58.3 | NC_005808.1 | + | 35860 | 0.66 | 0.462324 |
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Target: 5'- -uUCCGCUAUUGaGcGCGGcggcccagcGCAGCGGCu -3' miRNA: 3'- uuGGGCGAUGAC-CaCGCC---------CGUUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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