Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26702 | 3' | -55.6 | NC_005808.1 | + | 25746 | 0.74 | 0.186626 |
Target: 5'- gCGGCAGCAcgcugggcgcGCUGCUgGGCg-GCGGCAa -3' miRNA: 3'- -GUCGUCGU----------UGACGAgCUGagCGUCGU- -5' |
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26702 | 3' | -55.6 | NC_005808.1 | + | 33900 | 0.75 | 0.151462 |
Target: 5'- uCGGCAGCAAuCUGCgCGGCgcacaccugggcgCGCAGCAg -3' miRNA: 3'- -GUCGUCGUU-GACGaGCUGa------------GCGUCGU- -5' |
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26702 | 3' | -55.6 | NC_005808.1 | + | 495 | 0.76 | 0.148891 |
Target: 5'- uCGGCAGCAuACUGCgccgcggCGGCUUGCAGgCGg -3' miRNA: 3'- -GUCGUCGU-UGACGa------GCUGAGCGUC-GU- -5' |
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26702 | 3' | -55.6 | NC_005808.1 | + | 19694 | 1.08 | 0.000573 |
Target: 5'- gCAGCAGCAACUGCUCGACUCGCAGCAg -3' miRNA: 3'- -GUCGUCGUUGACGAGCUGAGCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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