miRNA: 3'- aGUaa-GCGCGGCC-GACCAGCGUUcuu -5'
miRNA: 3'- -AGUA---AGCGCGGCCGAC-C---AGCGUUCuu -5'
miRNA: 3'- aguaagCGCGGCCGACCag-CGU-UCUU- -5'
miRNA: 3'- -AGUAAgCGCGGCCGACCAGCGUUCUU- -5'
miRNA: 3'- aGUaa-GcGCGGCCGACCaGCGuUCUu -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26714 | 5' | -56.2 | NC_005808.1 | + | 38764 | 0.66 | 0.577667 |
|
Target: 5'- gCAaagUGCGCUGGaUUGGUCGCAAc-- -3' miRNA: 3'- aGUaa-GCGCGGCC-GACCAGCGUUcuu -5' |
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| 26714 | 5' | -56.2 | NC_005808.1 | + | 34117 | 0.71 | 0.2757 |
|
Target: 5'- uUCAUuacUCGCGUCGGCUGcGgcaUCGCAGGc- -3' miRNA: 3'- -AGUA---AGCGCGGCCGAC-C---AGCGUUCuu -5' |
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| 26714 | 5' | -56.2 | NC_005808.1 | + | 15366 | 0.67 | 0.490418 |
|
Target: 5'- -----gGCGCCGGCUGGacaGCAaAGGAu -3' miRNA: 3'- aguaagCGCGGCCGACCag-CGU-UCUU- -5' |
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| 26714 | 5' | -56.2 | NC_005808.1 | + | 11971 | 0.94 | 0.00593 |
|
Target: 5'- cUCAUU-GCGCCGGCUGGUCGCAAGAAc -3' miRNA: 3'- -AGUAAgCGCGGCCGACCAGCGUUCUU- -5' |
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| 26714 | 5' | -56.2 | NC_005808.1 | + | 11590 | 0.66 | 0.533409 |
|
Target: 5'- cCAgggC-CGCCGGCUGGcCGCcGGGc -3' miRNA: 3'- aGUaa-GcGCGGCCGACCaGCGuUCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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