Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26724 | 3' | -60.3 | NC_005808.1 | + | 24779 | 0.66 | 0.374497 |
Target: 5'- uGCCaCGGGUCGUcCUCgAUGCCCgcgUUGAa -3' miRNA: 3'- -CGG-GCUCGGCGuGGG-UACGGGa--AGCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 26046 | 0.66 | 0.357472 |
Target: 5'- aGCCCGcgcggcGGgCGCGCuCCAUGCgCUcggCGAa -3' miRNA: 3'- -CGGGC------UCgGCGUG-GGUACGgGAa--GCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 32625 | 0.67 | 0.332967 |
Target: 5'- gGCCgCGAcGCCGCGgUCGcUGUCCUgUCGAa -3' miRNA: 3'- -CGG-GCU-CGGCGUgGGU-ACGGGA-AGCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 39869 | 0.67 | 0.332967 |
Target: 5'- aGCgCCGGGCCGCGCUCAagcGUCCg---- -3' miRNA: 3'- -CG-GGCUCGGCGUGGGUa--CGGGaagcu -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 32115 | 0.67 | 0.325077 |
Target: 5'- cGCCCuGGCCGCugCCGaccucgacGCCCc-CGAa -3' miRNA: 3'- -CGGGcUCGGCGugGGUa-------CGGGaaGCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 18858 | 0.67 | 0.325077 |
Target: 5'- aGgCCGGGCg--GCCCAUGCCCacgUUCGGg -3' miRNA: 3'- -CgGGCUCGgcgUGGGUACGGG---AAGCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 11083 | 0.67 | 0.309717 |
Target: 5'- uGUCCGGGUCGCGCgCGaGCaCCUugUCGGc -3' miRNA: 3'- -CGGGCUCGGCGUGgGUaCG-GGA--AGCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 12919 | 0.69 | 0.253833 |
Target: 5'- cGCUCG-GCCGCGCCCAUaacgcuagucaGCUUcUCGGg -3' miRNA: 3'- -CGGGCuCGGCGUGGGUA-----------CGGGaAGCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 40155 | 0.7 | 0.21757 |
Target: 5'- cGCCUGGGCCGCAgUUUcgGCgCCUUCa- -3' miRNA: 3'- -CGGGCUCGGCGU-GGGuaCG-GGAAGcu -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 14703 | 0.7 | 0.21757 |
Target: 5'- gGCCUGcAGuuGCGCcuCCAUGCCCUgCGc -3' miRNA: 3'- -CGGGC-UCggCGUG--GGUACGGGAaGCu -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 38030 | 0.71 | 0.185831 |
Target: 5'- cGCaCCGAGCaGCGCCUcgGCgaCUUCGGc -3' miRNA: 3'- -CG-GGCUCGgCGUGGGuaCGg-GAAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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