Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26727 | 3' | -51.5 | NC_005808.1 | + | 91 | 0.69 | 0.663033 |
Target: 5'- aGCGAGCCAUuuCCAGaUCGUucugcgcgCCGUCc -3' miRNA: 3'- cCGCUCGGUAcuGGUUaAGCA--------GGUAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 323 | 0.66 | 0.814737 |
Target: 5'- gGGCGuGCCGU-ACCGGgggUCGUgCCAc- -3' miRNA: 3'- -CCGCuCGGUAcUGGUUa--AGCA-GGUag -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 922 | 0.66 | 0.860768 |
Target: 5'- cGGCauuGAGUguCAgggGGCCGAUcUGUCCGUCc -3' miRNA: 3'- -CCG---CUCG--GUa--CUGGUUAaGCAGGUAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 1693 | 1.14 | 0.000838 |
Target: 5'- cGGCGAGCCAUGACCAAUUCGUCCAUCg -3' miRNA: 3'- -CCGCUCGGUACUGGUUAAGCAGGUAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 3014 | 0.66 | 0.824407 |
Target: 5'- cGGCGGGUCAUaGCUgc-UCGUCCuUCc -3' miRNA: 3'- -CCGCUCGGUAcUGGuuaAGCAGGuAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 3799 | 0.68 | 0.741856 |
Target: 5'- cGGuCGGGCagCAUGACCAGgcggUCGggggcgCCGUUg -3' miRNA: 3'- -CC-GCUCG--GUACUGGUUa---AGCa-----GGUAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 3838 | 0.7 | 0.641191 |
Target: 5'- uGGUGAGUCAUGACCucggCGaaguuguaccaggcgCCGUCg -3' miRNA: 3'- -CCGCUCGGUACUGGuuaaGCa--------------GGUAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 4880 | 0.7 | 0.64004 |
Target: 5'- cGGCGAGCCAcGcaAUCAug-CGUCCuUCg -3' miRNA: 3'- -CCGCUCGGUaC--UGGUuaaGCAGGuAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 8613 | 0.67 | 0.763481 |
Target: 5'- uGGCGuAGgCGUGGCCGGUaCGcCCuUCg -3' miRNA: 3'- -CCGC-UCgGUACUGGUUAaGCaGGuAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 10084 | 0.66 | 0.833856 |
Target: 5'- gGGCGAGCCGUG-CCA-----UCC-UCg -3' miRNA: 3'- -CCGCUCGGUACuGGUuaagcAGGuAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 10635 | 0.7 | 0.594047 |
Target: 5'- cGCGuuCCGUGACCAGUUCGUaggcuUCg -3' miRNA: 3'- cCGCucGGUACUGGUUAAGCAggu--AG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 11423 | 0.69 | 0.674489 |
Target: 5'- cGGCGAGCCGgcGugCAggUCG-CCGa- -3' miRNA: 3'- -CCGCUCGGUa-CugGUuaAGCaGGUag -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 16321 | 0.74 | 0.391879 |
Target: 5'- aGCGAGUacgaCAUGGCCGAUUCGgugCCAc- -3' miRNA: 3'- cCGCUCG----GUACUGGUUAAGCa--GGUag -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 18027 | 0.67 | 0.804857 |
Target: 5'- cGGCccGaCCGUGGCCGGccUCG-CCAUCg -3' miRNA: 3'- -CCGcuC-GGUACUGGUUa-AGCaGGUAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 18465 | 0.74 | 0.401421 |
Target: 5'- cGCGGcGCCGgcggcgucGGCCGAUUCGUCCAc- -3' miRNA: 3'- cCGCU-CGGUa-------CUGGUUAAGCAGGUag -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 24570 | 0.66 | 0.824407 |
Target: 5'- uGGCGAgGCC--GGCCAcggUCGggCCGUCc -3' miRNA: 3'- -CCGCU-CGGuaCUGGUua-AGCa-GGUAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 24681 | 0.69 | 0.663033 |
Target: 5'- cGCGGGCCAcGuccacgcCCAGgcUGUCCAUCg -3' miRNA: 3'- cCGCUCGGUaCu------GGUUaaGCAGGUAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 28077 | 0.68 | 0.741856 |
Target: 5'- aGCGGGCagGUGGCCGcggcgUCGuauUCCGUCg -3' miRNA: 3'- cCGCUCGg-UACUGGUua---AGC---AGGUAG- -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 30640 | 0.66 | 0.814737 |
Target: 5'- cGGCGcgaaugagGGCgCA-GACCAAUUCGUCgGg- -3' miRNA: 3'- -CCGC--------UCG-GUaCUGGUUAAGCAGgUag -5' |
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26727 | 3' | -51.5 | NC_005808.1 | + | 30709 | 0.68 | 0.708542 |
Target: 5'- uGGCGGG-CAUGGCU--UUC-UCCAUCg -3' miRNA: 3'- -CCGCUCgGUACUGGuuAAGcAGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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