miRNA: 3'- -CCGC--------UCGGUACUGGUUaagCaGGUAG- -5'
miRNA: 3'- -CCGCUCGGUACuGGUuaagcAGGuAG- -5'
miRNA: 3'- -CCG---CUCG--GUa--CUGGUUAaGCAGGUAG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26727 | 3' | -51.5 | NC_005808.1 | + | 41879 | 0.66 | 0.824407 |
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Target: 5'- aGGCGcucgacuuGGCCGUGGCCGAcaaGaUCAUCa -3' miRNA: 3'- -CCGC--------UCGGUACUGGUUaagCaGGUAG- -5' |
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| 26727 | 3' | -51.5 | NC_005808.1 | + | 10084 | 0.66 | 0.833856 |
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Target: 5'- gGGCGAGCCGUG-CCA-----UCC-UCg -3' miRNA: 3'- -CCGCUCGGUACuGGUuaagcAGGuAG- -5' |
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| 26727 | 3' | -51.5 | NC_005808.1 | + | 922 | 0.66 | 0.860768 |
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Target: 5'- cGGCauuGAGUguCAgggGGCCGAUcUGUCCGUCc -3' miRNA: 3'- -CCG---CUCG--GUa--CUGGUUAaGCAGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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