Results 1 - 20 of 57 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26734 | 3' | -60.5 | NC_005808.1 | + | 40366 | 0.68 | 0.287725 |
Target: 5'- -cGGCCacGCgCCGCCUgcUGCGCGUgGUGc -3' miRNA: 3'- cuUCGG--CG-GGUGGA--ACGCGCGgCACc -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 33273 | 0.74 | 0.10777 |
Target: 5'- --cGCCGCCCGCCgcgccgacGUGCGCCGccaGGu -3' miRNA: 3'- cuuCGGCGGGUGGaa------CGCGCGGCa--CC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 29156 | 0.72 | 0.154025 |
Target: 5'- cGAGGCCGCCaAgCUgGCGggccUGCCGUGGg -3' miRNA: 3'- -CUUCGGCGGgUgGAaCGC----GCGGCACC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 19061 | 0.69 | 0.253833 |
Target: 5'- cGAGGCCaucgaccgcguGCgCCGCCUggaagGCGCGCuCGcGGa -3' miRNA: 3'- -CUUCGG-----------CG-GGUGGAa----CGCGCG-GCaCC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 1762 | 0.68 | 0.260339 |
Target: 5'- -uGGCCgGCCUgcGCCUgGCGCGUCG-GGc -3' miRNA: 3'- cuUCGG-CGGG--UGGAaCGCGCGGCaCC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 35496 | 0.71 | 0.162572 |
Target: 5'- --cGCaGUCCAUCgugcgcguggGCGCGCCGUGGg -3' miRNA: 3'- cuuCGgCGGGUGGaa--------CGCGCGGCACC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 18594 | 0.7 | 0.211975 |
Target: 5'- -uGGCCgacgcGCCCACCUucgccagucUGUGCGCCGa-- -3' miRNA: 3'- cuUCGG-----CGGGUGGA---------ACGCGCGGCacc -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 8688 | 0.69 | 0.235115 |
Target: 5'- --cGCCGCCCAgg-UGUGCGCCGc-- -3' miRNA: 3'- cuuCGGCGGGUggaACGCGCGGCacc -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 41884 | 0.68 | 0.285595 |
Target: 5'- aAAGCCGCCCcuauccugggcgguAUCUUGgGCGCCc-GGc -3' miRNA: 3'- cUUCGGCGGG--------------UGGAACgCGCGGcaCC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 34381 | 0.67 | 0.309717 |
Target: 5'- -cGGCCGCCgACCa-GCGCgacuacgagGCCGUGc -3' miRNA: 3'- cuUCGGCGGgUGGaaCGCG---------CGGCACc -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 18384 | 0.66 | 0.392064 |
Target: 5'- -cGGCCGCUCGCCggacuuugGCaGCGCCuacGUGc -3' miRNA: 3'- cuUCGGCGGGUGGaa------CG-CGCGG---CACc -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 15279 | 0.7 | 0.190822 |
Target: 5'- aAAGCaaCGCgCGCCUgUGgGCGUCGUGGg -3' miRNA: 3'- cUUCG--GCGgGUGGA-ACgCGCGGCACC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 29207 | 0.71 | 0.180955 |
Target: 5'- cGAuGCCGCCUACaaccGCGCGCUG-GGc -3' miRNA: 3'- -CUuCGGCGGGUGgaa-CGCGCGGCaCC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 20305 | 0.68 | 0.260339 |
Target: 5'- --uGCUGCCCGaCUU-CGaCGCCGUGGu -3' miRNA: 3'- cuuCGGCGGGUgGAAcGC-GCGGCACC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 19481 | 0.68 | 0.280672 |
Target: 5'- aGGAGCCGUuaccgcugUCGCCguggGCGCcGCCGuUGGc -3' miRNA: 3'- -CUUCGGCG--------GGUGGaa--CGCG-CGGC-ACC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 7259 | 0.7 | 0.211975 |
Target: 5'- cGAuGUCGCCgGCCUUGUGCagcuucucgGCgGUGGu -3' miRNA: 3'- -CUuCGGCGGgUGGAACGCG---------CGgCACC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 16845 | 0.67 | 0.302247 |
Target: 5'- --cGCCGCCCAgCa-GCGCGCCcagcGUGc -3' miRNA: 3'- cuuCGGCGGGUgGaaCGCGCGG----CACc -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 34754 | 0.67 | 0.317327 |
Target: 5'- cGAAGcCCGCCuCACCau-CGCGCUGaacaUGGg -3' miRNA: 3'- -CUUC-GGCGG-GUGGaacGCGCGGC----ACC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 16553 | 0.69 | 0.221561 |
Target: 5'- uGGAGCgCGCCCGCCgcGCGggcuuugaugccauCGCCGaGGa -3' miRNA: 3'- -CUUCG-GCGGGUGGaaCGC--------------GCGGCaCC- -5' |
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26734 | 3' | -60.5 | NC_005808.1 | + | 14023 | 0.68 | 0.260339 |
Target: 5'- --cGCUGCCggugGCCgacgUGCGCGCCGcgaUGGc -3' miRNA: 3'- cuuCGGCGGg---UGGa---ACGCGCGGC---ACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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