Results 61 - 68 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26740 | 3' | -55.8 | NC_005808.1 | + | 7311 | 0.73 | 0.251549 |
Target: 5'- gGCGCcgucgauGGUCuGCGAacGCAGCAgCCGGu -3' miRNA: 3'- aCGCG-------UCAGuCGCUaaCGUCGU-GGCC- -5' |
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26740 | 3' | -55.8 | NC_005808.1 | + | 24555 | 0.73 | 0.226796 |
Target: 5'- cGCGCAGcgCGGCGAUgGCGagGCCGGc -3' miRNA: 3'- aCGCGUCa-GUCGCUAaCGUcgUGGCC- -5' |
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26740 | 3' | -55.8 | NC_005808.1 | + | 29600 | 0.74 | 0.220791 |
Target: 5'- cGCGCGGUUuGCcaGUUGUccAGCGCCGGg -3' miRNA: 3'- aCGCGUCAGuCGc-UAACG--UCGUGGCC- -5' |
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26740 | 3' | -55.8 | NC_005808.1 | + | 8663 | 0.74 | 0.198112 |
Target: 5'- cGCGCGGUCGGC-AUUGC-GUGCCGc -3' miRNA: 3'- aCGCGUCAGUCGcUAACGuCGUGGCc -5' |
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26740 | 3' | -55.8 | NC_005808.1 | + | 19069 | 0.74 | 0.192768 |
Target: 5'- aGCGUguccucgccgaGGUCAcGCGAUUGCAG-GCCGGc -3' miRNA: 3'- aCGCG-----------UCAGU-CGCUAACGUCgUGGCC- -5' |
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26740 | 3' | -55.8 | NC_005808.1 | + | 4250 | 0.76 | 0.145966 |
Target: 5'- gGCGUAGUCGGUGccgGCcgucAGCGCCGGc -3' miRNA: 3'- aCGCGUCAGUCGCuaaCG----UCGUGGCC- -5' |
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26740 | 3' | -55.8 | NC_005808.1 | + | 1463 | 0.79 | 0.092255 |
Target: 5'- gGCGCGGUCGGCGGccuccugGCAG-GCCGGg -3' miRNA: 3'- aCGCGUCAGUCGCUaa-----CGUCgUGGCC- -5' |
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26740 | 3' | -55.8 | NC_005808.1 | + | 7749 | 1.1 | 0.000538 |
Target: 5'- cUGCGCAGUCAGCGAUUGCAGCACCGGc -3' miRNA: 3'- -ACGCGUCAGUCGCUAACGUCGUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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