Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26748 | 3' | -50.3 | NC_005808.1 | + | 10885 | 0.66 | 0.845544 |
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Target: 5'- ---aAGCCCGCGCgCGGCgggcUGGUCAg -3' miRNA: 3'- uuuaUUGGGCGUGgGUUGa---GCUAGUg -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 33782 | 0.66 | 0.863483 |
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Target: 5'- --cUGGCCCG-ACCCAGCa-GAUCGg -3' miRNA: 3'- uuuAUUGGGCgUGGGUUGagCUAGUg -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 42185 | 0.66 | 0.863483 |
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Target: 5'- -cGUGGCCCGCgacgugcuGCUCAACagUCGcgCGCu -3' miRNA: 3'- uuUAUUGGGCG--------UGGGUUG--AGCuaGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 41039 | 0.66 | 0.863483 |
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Target: 5'- aGGAUAugCC-CAUCCAGC-CGGUCGa -3' miRNA: 3'- -UUUAUugGGcGUGGGUUGaGCUAGUg -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 28489 | 0.66 | 0.863483 |
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Target: 5'- cGAgcAUCCGCucggcauuGCCgAACUCGGUCAUc -3' miRNA: 3'- uUUauUGGGCG--------UGGgUUGAGCUAGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 9999 | 0.66 | 0.872044 |
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Target: 5'- --uUGACgCGgG-CCAGCUUGGUCACa -3' miRNA: 3'- uuuAUUGgGCgUgGGUUGAGCUAGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 32083 | 0.66 | 0.88032 |
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Target: 5'- -cGUGGCCgGCGgUCAGCaguUCGAUCAa -3' miRNA: 3'- uuUAUUGGgCGUgGGUUG---AGCUAGUg -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 22286 | 0.66 | 0.888303 |
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Target: 5'- ---cAGCCCGCAUCgCAAC-CGGcCGCc -3' miRNA: 3'- uuuaUUGGGCGUGG-GUUGaGCUaGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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