Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26748 | 3' | -50.3 | NC_005808.1 | + | 12745 | 0.71 | 0.614735 |
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Target: 5'- ---gGugCCGCGCCCAgacGCUUGG-CGCg -3' miRNA: 3'- uuuaUugGGCGUGGGU---UGAGCUaGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 11368 | 0.68 | 0.764476 |
|
Target: 5'- ---cGACCUGCucGCCCGGCaccUCGGUgGCg -3' miRNA: 3'- uuuaUUGGGCG--UGGGUUG---AGCUAgUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 10885 | 0.66 | 0.845544 |
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Target: 5'- ---aAGCCCGCGCgCGGCgggcUGGUCAg -3' miRNA: 3'- uuuaUUGGGCGUGgGUUGa---GCUAGUg -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 9999 | 0.66 | 0.872044 |
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Target: 5'- --uUGACgCGgG-CCAGCUUGGUCACa -3' miRNA: 3'- uuuAUUGgGCgUgGGUUGAGCUAGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 7465 | 0.69 | 0.731143 |
|
Target: 5'- cGAAUGGCCuUGCACCagGACUCGAcCAg -3' miRNA: 3'- -UUUAUUGG-GCGUGGg-UUGAGCUaGUg -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 5027 | 0.69 | 0.70833 |
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Target: 5'- ---cAGCgCCGCgauGCCCcGCUCGAUgGCg -3' miRNA: 3'- uuuaUUG-GGCG---UGGGuUGAGCUAgUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 3079 | 0.66 | 0.845544 |
|
Target: 5'- ---cGAgCCGCACCCAugcccUUCGA-CACg -3' miRNA: 3'- uuuaUUgGGCGUGGGUu----GAGCUaGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 1145 | 0.71 | 0.591264 |
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Target: 5'- --uUAGCUCGCGCUaCGGCggCGGUCACg -3' miRNA: 3'- uuuAUUGGGCGUGG-GUUGa-GCUAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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