Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26748 | 3' | -50.3 | NC_005808.1 | + | 25264 | 0.7 | 0.638275 |
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Target: 5'- ----uGCUCGCGCU--GCUCGGUCACu -3' miRNA: 3'- uuuauUGGGCGUGGguUGAGCUAGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 33865 | 0.7 | 0.661789 |
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Target: 5'- cGGUAGCgCCGCGCCC-GC-CGggCACa -3' miRNA: 3'- uUUAUUG-GGCGUGGGuUGaGCuaGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 32610 | 0.69 | 0.70718 |
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Target: 5'- --cUGGCCCGCgucaaggGCCgCGACgccgCGGUCGCu -3' miRNA: 3'- uuuAUUGGGCG-------UGG-GUUGa---GCUAGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 11368 | 0.68 | 0.764476 |
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Target: 5'- ---cGACCUGCucGCCCGGCaccUCGGUgGCg -3' miRNA: 3'- uuuaUUGGGCG--UGGGUUG---AGCUAgUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 18135 | 0.68 | 0.775295 |
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Target: 5'- ---gAACuuGuCGCCCGGCUCGcgCAg -3' miRNA: 3'- uuuaUUGggC-GUGGGUUGAGCuaGUg -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 18541 | 0.67 | 0.806681 |
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Target: 5'- --uUGGCCUG-GCCgAAgUCGAUCACg -3' miRNA: 3'- uuuAUUGGGCgUGGgUUgAGCUAGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 28489 | 0.66 | 0.863483 |
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Target: 5'- cGAgcAUCCGCucggcauuGCCgAACUCGGUCAUc -3' miRNA: 3'- uUUauUGGGCG--------UGGgUUGAGCUAGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 16982 | 1.07 | 0.002814 |
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Target: 5'- aAAAUAACCCGCACCCAACUCGAUCACc -3' miRNA: 3'- -UUUAUUGGGCGUGGGUUGAGCUAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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