Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26770 | 5' | -60 | NC_005808.1 | + | 17834 | 0.66 | 0.438584 |
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Target: 5'- uCGGGCGGGUUgauGC-CCGCCaCCguggCCUCg -3' miRNA: 3'- -GCUUGCCCAGau-CGuGGUGG-GG----GGGG- -5' |
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| 26770 | 5' | -60 | NC_005808.1 | + | 34966 | 0.67 | 0.392711 |
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Target: 5'- gGcgUGGGUCga--GCCGCgCCCCCUg -3' miRNA: 3'- gCuuGCCCAGaucgUGGUGgGGGGGG- -5' |
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| 26770 | 5' | -60 | NC_005808.1 | + | 9989 | 0.67 | 0.373539 |
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Target: 5'- uGAGCguggcgGGGUCgaaagugccgcGCGCCcgGCCCCCgCCCu -3' miRNA: 3'- gCUUG------CCCAGau---------CGUGG--UGGGGG-GGG- -5' |
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| 26770 | 5' | -60 | NC_005808.1 | + | 33584 | 0.67 | 0.358332 |
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Target: 5'- aCGAACuGGU---GCGCCGCCUgcuggaagcgCCCCCg -3' miRNA: 3'- -GCUUGcCCAgauCGUGGUGGG----------GGGGG- -5' |
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| 26770 | 5' | -60 | NC_005808.1 | + | 23937 | 0.69 | 0.268276 |
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Target: 5'- aGGcCGGGggcgGGCGCCGCCCCCgaucacgaaUCCa -3' miRNA: 3'- gCUuGCCCaga-UCGUGGUGGGGG---------GGG- -5' |
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| 26770 | 5' | -60 | NC_005808.1 | + | 35645 | 0.73 | 0.139587 |
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Target: 5'- cCGAGCGGGUCaAGCGUCAauaCCCCCa -3' miRNA: 3'- -GCUUGCCCAGaUCGUGGUgggGGGGG- -5' |
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| 26770 | 5' | -60 | NC_005808.1 | + | 17226 | 0.74 | 0.128651 |
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Target: 5'- aGGACGcGGacgUUGGaCAUCACCCCCUCCa -3' miRNA: 3'- gCUUGC-CCa--GAUC-GUGGUGGGGGGGG- -5' |
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| 26770 | 5' | -60 | NC_005808.1 | + | 29874 | 1.1 | 0.000234 |
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Target: 5'- gCGAACGGGUCUAGCACCACCCCCCCCc -3' miRNA: 3'- -GCUUGCCCAGAUCGUGGUGGGGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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