miRNA: 3'- cUUGGGCCgCGCg--AGCGUGUUGAUac -5'
miRNA: 3'- cUUGGGCCGCGCga--GCGuGUUGAUac -5'
miRNA: 3'- cUUGG-GCCGCGCGa---GCgUGUUGAuac -5'
miRNA: 3'- cuUGGGCCG-CGCGAG-----CGUGUUGaUAC- -5'
miRNA: 3'- cUUGGGCCGCGCgAGCGugUU--GAUAC- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26775 | 3' | -55.4 | NC_005808.1 | + | 38006 | 0.67 | 0.526411 |
|
Target: 5'- uGACCUGG-GCGgcaUCGCAUAACUAg- -3' miRNA: 3'- cUUGGGCCgCGCg--AGCGUGUUGAUac -5' |
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| 26775 | 3' | -55.4 | NC_005808.1 | + | 38211 | 0.68 | 0.46301 |
|
Target: 5'- aGGCCCGGCGCGacggCGCcUGGCUGc- -3' miRNA: 3'- cUUGGGCCGCGCga--GCGuGUUGAUac -5' |
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| 26775 | 3' | -55.4 | NC_005808.1 | + | 38440 | 0.66 | 0.615296 |
|
Target: 5'- cGACCaCGGCGCGCaaggUGgGCGGCUu-- -3' miRNA: 3'- cUUGG-GCCGCGCGa---GCgUGUUGAuac -5' |
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| 26775 | 3' | -55.4 | NC_005808.1 | + | 38559 | 0.74 | 0.219437 |
|
Target: 5'- -cGCCCGGCuGCGCUCgacccGCGCGGC-AUGa -3' miRNA: 3'- cuUGGGCCG-CGCGAG-----CGUGUUGaUAC- -5' |
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| 26775 | 3' | -55.4 | NC_005808.1 | + | 39023 | 0.74 | 0.231577 |
|
Target: 5'- uGACCCGGCGCG-UCGCugAAauUUAUGc -3' miRNA: 3'- cUUGGGCCGCGCgAGCGugUU--GAUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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