miRNA: 3'- -CUUGGGCCGCGCGAG-CGU-GU-UGA--UAC- -5'
miRNA: 3'- cuUGGGCCGCGCGAgCgUGUU-GAUAC- -5'
miRNA: 3'- cuUGGG----CCGCGCGAGCGUgUUGAUac -5'
miRNA: 3'- -CUUGGGCCGCGCGAG-CGUGUUGauac -5'
miRNA: 3'- -CUUGGGCCGCGCGAGCGUGUUGAUAC- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26775 | 3' | -55.4 | NC_005808.1 | + | 28312 | 0.74 | 0.202251 |
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Target: 5'- aGAACUCGGCGCGCUCgGCGuCGgACUcgAUGg -3' miRNA: 3'- -CUUGGGCCGCGCGAG-CGU-GU-UGA--UAC- -5' |
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| 26775 | 3' | -55.4 | NC_005808.1 | + | 36860 | 0.75 | 0.171367 |
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Target: 5'- -uGCCCGGCGCGCU-GgGCGAuCUGUGc -3' miRNA: 3'- cuUGGGCCGCGCGAgCgUGUU-GAUAC- -5' |
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| 26775 | 3' | -55.4 | NC_005808.1 | + | 19083 | 0.75 | 0.171367 |
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Target: 5'- -cGCCUggaaGGCGCGCUCGCGgAACUGc- -3' miRNA: 3'- cuUGGG----CCGCGCGAGCGUgUUGAUac -5' |
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| 26775 | 3' | -55.4 | NC_005808.1 | + | 20270 | 0.79 | 0.105594 |
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Target: 5'- aGAACUCGGCGCGUUCgGCACGGCc--- -3' miRNA: 3'- -CUUGGGCCGCGCGAG-CGUGUUGauac -5' |
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| 26775 | 3' | -55.4 | NC_005808.1 | + | 32043 | 1.07 | 0.000876 |
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Target: 5'- uGAACCCGGCGCGCUCGCACAACUAUGc -3' miRNA: 3'- -CUUGGGCCGCGCGAGCGUGUUGAUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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