Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26807 | 5' | -54.7 | NC_005808.1 | + | 23931 | 0.71 | 0.353723 |
Target: 5'- -uGUAACAGGcCGGGGGCGGGCgccGCc -3' miRNA: 3'- guCGUUGUCUaGCUCCUGUCCGa--CGc -5' |
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26807 | 5' | -54.7 | NC_005808.1 | + | 24082 | 0.67 | 0.610923 |
Target: 5'- gCGGCGACcGcgCGGGGcACGGGCaccccgcuacaUGCGc -3' miRNA: 3'- -GUCGUUGuCuaGCUCC-UGUCCG-----------ACGC- -5' |
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26807 | 5' | -54.7 | NC_005808.1 | + | 27475 | 0.67 | 0.599603 |
Target: 5'- cCAGCAugGGGUCGGGccuGGUAGGCaGCu -3' miRNA: 3'- -GUCGUugUCUAGCUC---CUGUCCGaCGc -5' |
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26807 | 5' | -54.7 | NC_005808.1 | + | 27817 | 0.69 | 0.489483 |
Target: 5'- cCAGCGacaucgugaaGCAGAUCGAcaccGACAcGCUGCGc -3' miRNA: 3'- -GUCGU----------UGUCUAGCUc---CUGUcCGACGC- -5' |
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26807 | 5' | -54.7 | NC_005808.1 | + | 28056 | 0.68 | 0.521704 |
Target: 5'- uCGGCGACGGucugcUCGAacagcGGGCAGGUggccGCGg -3' miRNA: 3'- -GUCGUUGUCu----AGCU-----CCUGUCCGa---CGC- -5' |
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26807 | 5' | -54.7 | NC_005808.1 | + | 32476 | 0.66 | 0.65629 |
Target: 5'- aGGCGugGccGGUaCGAGGACAGGCa--- -3' miRNA: 3'- gUCGUugU--CUA-GCUCCUGUCCGacgc -5' |
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26807 | 5' | -54.7 | NC_005808.1 | + | 32930 | 0.67 | 0.610923 |
Target: 5'- aCGGCGGCuGuUCGgccAGGGCGaccacGGCUGCGu -3' miRNA: 3'- -GUCGUUGuCuAGC---UCCUGU-----CCGACGC- -5' |
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26807 | 5' | -54.7 | NC_005808.1 | + | 34046 | 0.68 | 0.532629 |
Target: 5'- cCAGgAugAGGUCGAGcGCGGcCUGCGa -3' miRNA: 3'- -GUCgUugUCUAGCUCcUGUCcGACGC- -5' |
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26807 | 5' | -54.7 | NC_005808.1 | + | 34360 | 0.69 | 0.489483 |
Target: 5'- cCAGCGACuucgccGA-CGAGGGCAcGGCcGCGc -3' miRNA: 3'- -GUCGUUGu-----CUaGCUCCUGU-CCGaCGC- -5' |
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26807 | 5' | -54.7 | NC_005808.1 | + | 35385 | 0.66 | 0.6676 |
Target: 5'- gCGGC-GCAGAUgcugcCGuGGACAucgcgcugucccGGCUGCGu -3' miRNA: 3'- -GUCGuUGUCUA-----GCuCCUGU------------CCGACGC- -5' |
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26807 | 5' | -54.7 | NC_005808.1 | + | 40256 | 1.08 | 0.000885 |
Target: 5'- cCAGCAACAGAUCGAGGACAGGCUGCGc -3' miRNA: 3'- -GUCGUUGUCUAGCUCCUGUCCGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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